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不同方法检测低水平HBsAg的结果对比分析

The comparative analysis of different methods for the detection of low levels of HBs Ag results

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【作者】 赵凯

【Author】 ZHAO Kai;Laboratory Department of Shangqiu First People’s Hospital of Henan;

【机构】 商丘市第一人民医院

【摘要】 目的:比较酶联免疫吸附试验(ELISA)和全自动微粒子免疫荧光法(MEIA)在低水平乙型肝炎病毒表面抗原(HBs Ag)检测中的应用价值。方法:先用MEIA法检测乙型肝炎表面抗原,从中筛选出的112例低水平HBs Ag阳性标本,再用ELISA法进行检测,并对结果进行对比评价。结果:MEIA法检测结果为2<S/N<5时,ELISA法的阳性率仅为12.77%;5<S/N<10时,ELISA法的阳性率仅为35.29%;ELISA法与MEIA法两种方法阳性检出率差异有统计学意义(P<0.05);10<S/N<20时,阳性检出率差异无统计学意义(P>0.05)。结论:MEIA法对低水平HBs Ag的阳性检出率比ELISA法高。

【Abstract】 Objective: To compare the enzyme linked immunosorbent assay(ELISA)and microparticle immunofluorescence assay(MEIA) in the low level of hepatitis Bvirus surface antigen(HBs Ag) and application value in the detection of. Methods: The first detection of hepatitis B surface antigen by MEIA method, 112 patients withlow levels of HBsA g positive samples screened out, and then the ELISA method detection, and compared to the results of evaluation. Results: MEIA assay results for 2 < S/N < 5, the positive rate of ELISA was only 12.77%; 5 < S/N < 10, the positive rate of ELISA was only 35.29%; the ELISA method and MEIA method twopositive rate were significantly different between(P< 10 < S/N <0.05); 20, the positive rate showed no statistical significance(P> 0.05). Conclusion: MEIA on lowlevel of HBs Ag positive rate is higher than ELISA method.

  • 【文献出处】 临床医药文献电子杂志 ,Journal of Clinical Medical Literature , 编辑部邮箱 ,2014年04期
  • 【分类号】R446.6
  • 【下载频次】16
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