【摘要】 目的 从DNA、RNA和蛋白水平分析胃癌中E-cadherin(E-cad)表达,旨在探讨E-cad在胃癌侵袭转移中的作用及对预后的影响。方法 分别应用免疫组化SP法及原位杂交检测60例胃癌组织和癌旁组织中的E-cad蛋白表达及E-cad mRNA表达;同时应用MSP法检测E-cad DNA甲基化水平。结果 E-cad mRNA和E-cad蛋白在胃癌中的阳性表达显著低于癌旁组织,E-cad DNA甲基化在胃癌阳性表达率高于癌旁组织。E-cad DNA甲基化与E-cad蛋白、E-cad mRNA在胃癌中的表达缺失呈负相关性(r=-0.335,r=-0.271),E-cad基因甲基化与胃癌5年生存率成负相关(r=-0.381,P<0.01)。E-cad蛋白表达与胃癌5年生存率成正相关,(r=0.378,P<0.01)。结论 E-cad低表达是胃癌的重要生物学特征,而E-cad甲基化可能是导致胃癌E-cad mRNA和Ecad蛋白低表达的重要原因,E-cad甲基化水平可以作为预测胃癌侵袭转移及预后的重要指标。更多还原

【Abstract】 Objective To investigate the role and prognostic impact of E-cadherin(E-cad) on invasion and metastasis of gastric carcinoma by analyzing E-cadherin expression at DNA,RNA and protein levels.Methods RNA and protein expression of E-cad were tested in 60 cases of gastric carcinoma and adjacent tissues by streptavidin peroxidase(SP) immunohistochemistry and in situ hybridization.DNA methylation of E-cad was tested with methylation-specific polymerase chain reaction(MSP).Results The positive rates of E-cadherin protein and mRNA in gastric carcinoma were significantly lower than those in adjacent tissues.DNA methylation of E-cad negatively correlated with the E-cad protein and RNA expression in gastric carcinoma respectively(r =-0.355,r =- 0.271).DNA methylation of E-cad negatively correlated with the 5-year survival rate of gastric carcinoma(r =- 0.381,P < 0.01).However,E-cad protein positively correlated with the 5-year survival rate of gastric carcinoma(r =0.378,P <0.01).Conclusion The decreased expression of E-cad is one of the most important biological features in gastric carcinoma.Methylation of E-cad DNA may be an important agent causing low expression of E-cad protein and mRNA and it can be used as an important indicator in predicting invasion and metastasis of gastric cancer.更多还原