【摘要】 目的:探讨加味导赤散与维生素B12联合治疗复发性口腔溃疡(ROU)动物模型的作用及其对血清IL-6及TNF-α的影响。方法:采用免疫方法建立ROU动物模型。48只SD大鼠随机分为正常组、模型组、左旋咪唑组(对照组)、低剂量加味导赤散加维生素B12组(治疗组1)、中剂量加味导赤散加维生素B12组(治疗组2)、高剂量加味导赤散加维生素B12组(治疗组3),每组8只。对照组用左旋咪唑片灌胃,治疗组分别用低、中、高剂量的加味导赤散与维生素B12一起灌胃,观察记录溃疡变化情况,用酶联免疫法(ELISA)测定大鼠造模前后及灌胃后血清IL-6及TNF-α含量。结果:1.实验大鼠于第3次注射抗原乳化剂1周后开始出现口腔溃疡。2.对照组与治疗组2、组3的大鼠口腔溃疡分别较模型组发生数目明显减少,持续时间明显缩短,间隔时间明显延长(P<0.05)。3.模型组IL-6及TNF-α水平较正常组明显升高(P<0.05),而对照组与2、3治疗组较溃疡组下降(P<0.05)。结论:加味导赤散加维生素B12可能通过下调IL-6及TNF-α水平促进口腔溃疡愈合,减少溃疡复发。更多还原

【Abstract】 Objective:1.Establish the rat’s recurrent oral ulceration(ROU)models;2.To evaluate the therapeutic effects of traditional chinese medicine Dao chi San plus gypsum decoction(DCSGd)with vitamin B12 on experimental oral ulcer. Method:Experimental animal model of ROU was established by immunological method(by injecting antigens into SD rats). 48 rats were randomized into six groups,including normal group,ulcer group(model group),Levamisole group,lowdose DCSGd plus vitamin B12group(therapy group 1),medium-dose DCSGd plus vitamin B12group(therapy group 2),and high-dose DCSGd plus vitamin B12group(therapy group 3). All groups were taken orally. During these days,observe general symptoms and the oral ulcers change of rats in each group. The therapeutic effects were observed and the level of IL-6 and TNF-α were measured in different groups. Result:1.the ulcers were formed one week after the third injection.2.Compared with ulcer group,the number of ulcers are reduced,interval are increased and the duration are shorten in Levamisole group,therapy group 2 and therapy group 3. 3.Compared with normal group,the expression intensity of IL-6 and TNF-α in model group was significantly higher(P <0.05). The level of IL-6 and TNF-α were decreased in Levamisole group,therapy group 2and therapy group 3,compared with model group(P <0.05). Conclusion:The abnormal increase of IL-6 and TNF-α is related to ROU. DCSGd plus vitamin B12 can promote ulcer healing,reduce symptoms and reduce ulcer recurrence.更多还原