【摘要】 目的探讨苦参碱对体外培养人肾母细胞瘤SK-NEP-1细胞抑制增殖及诱导凋亡的作用。方法体外培养人肾母细胞瘤SK-NEP-1细胞分对照组,0.5 mg/ml、1.0 mg/ml、1.5 mg/ml苦参碱干预组,应用四甲基偶氮蓝比色法、流式细胞术检测苦参碱对SK-NEP-1细胞抑制率、凋亡率的影响,逆转录聚合酶链反应检测SK-NEP-1细胞的趋化因子受体4(CXCR4)mRNA表达。结果不同浓度苦参碱干预组及对照组SK-NEP-1细胞的抑制率、凋亡率以及CXCR4 mRNA表达差异均有统计学意义(P<0.01);随苦参碱浓度增加,SK-NEP-1细胞的抑制率和凋亡率呈上升趋势,而CXCR4 mRNA表达呈下降趋势,差异均有统计学意义(P<0.01)。结论苦参碱可以抑制SK-NEP-1细胞增殖并诱导凋亡,该作用可能与下调CXCR4的表达有关。更多还原

【Abstract】 Objectives To investigate the effects of matrine on the proliferation and apoptosis of SK-NEP-1 cells in vitro, and its possible mechanism. Methods Trials were divided into following groups: control group, 0.5, 1.0 and 1.5mg/ml of matrine intervention groups. The inhibition rate of SK-NEP-1 cells treated with different concentration of Matrine was detected by MTT colorimetric assay. Apoptosis rate was detected by flow cytometry(FCM). RT-PCR analysis was employed to measure the PDCD4 mRNA expression. Results Matrine(final concentrations=0.5, 1.0 and 1.5mg/ml) could induce apoptosis and inhibit the growth of SK-NEP-1 cells. Compared with the controls without matrine treatment(8.81±3.71) %, the inhibition rates of SKNEP-1 cells were(20.79±6.20)%,(31.25±5.07)%, and(51.15±12.70)%, respectively; the apoptotic rates of SK-NEP-1 cells treated with different concentration of matrine were(13.67±0.78)%,(17.43±1.65)% and(20.80±1.54)%, respectively. Significant difference in the inhibition and apoptotic rates of SK-NEP-1 cells between each drug group and control group was observed(P< 0.05), and the inhibition and apoptotic rates of SK-NEP-1 cells increased gradually with increased matrine concentration, thus exhibiting a dose-dependent effect(P<0.05). To the expression of CXCR4 mRNA,the grey levels of SK-NEP-1 cells treated with matrine intervention group(final concentrations=0.5, 1.0 and 1.5 mg/ml) were(0.720±0.058),(0.540±0.095) and(0.307± 0.050), respectively. The mRNA expression of CXCR4 was seen in SK-NEP-1 cells. Compared with control group, the expression of CXCR4 mRNA was decreased significantly in matrine intervention group(P<0.01).There were significant difference in CXCR4 mRNA level among the SK- NEP- 1 cells treated with 0.5,1.0,1.5mg/mL of matrine(P<0.01). Conclusions Matrine could induce apoptosis and inhibit the growth of SK-NEP-1 cells in a dose-dependent way which may be associated with the down-regulated CXCR4 expression in SK-NEP-1 cells.更多还原