【摘要】 目的研究白细胞介素(IL)-17对系膜细胞增殖及分泌功能的影响,并探讨其可能的分子机制。方法四甲基偶氮唑盐比色(MTT)法检测IL-17对系膜细胞增殖的影响;酶联免疫法(ELISA)检测IL-17对系膜细胞分泌IL-1β的影响;免疫细胞化学法检测系膜细胞NF-κB的表达。结果不同浓度IL-17在不同时间点对系膜细胞增殖的影响均无统计学意义(P>0.05);IL-17可促进系膜细胞分泌IL-1β,且呈浓度时间依赖性,差异有统计学意义(P<0.05);同时IL-17诱导组NF-κB的表达高于空白对照组,差异有统计学意义(P<0.05)。结论 IL-17可能部分通过NF-κB途径引起系膜细胞分泌炎症因子IL-1β,加重肾脏局部的炎症状态,导致肾脏损伤。更多还原

【Abstract】 Objectives To investigate the effect of IL-17 on proliferation and secretion of IL-1β in the human mesangial cell line(HMCL) and explore the molecular mechanism. Methods The proliferation of HMCL induced by IL-17 was detected by MTT, the secretion of IL-1β in HMCL was measured by ELISA and the expression of NF-κB was detected by immunocytochemistry. Results IL-17 did not impact HMCL proliferation(P>0.05) but dose-and time-dependently promoted the secretion of IL-1β(P<0.05). And the expression of NF-κB induced by IL-17 increased comparing to that in the control group(P<0.05). Conclusions IL-17 may induce HMCL to secrete IL-1β via NF-κB pathway that results in kidney injury.更多还原