【摘要】 富勒烯纳米材料因其独特的结构和生物活性在生物医学领域广受关注,然而真正实现它们的商业化应用,仍需要进一步地研究,解决关键的生物安全性问题.本文利用电弧放电法,以稳定同位素13C直接替位掺入C60碳笼上的骨架碳原子,合成了13C-C60,确定了稳定同位素13C的掺入量,研究了稳定同位素效应所引起的C60分子质谱及振动光谱的变化特征,证实这种13C直接标记法不破坏富勒烯碳笼的本征结构.研究发现13C标记的C60分子的质谱分子离子峰呈正态分布,较强的分子离子峰m/z随13C标记量的增加而增大;其分子振动的红外和拉曼光谱虽然都保留了C60分子的特征光谱峰,但又随13C标记量的增加而发生不同程度地迁移和劈裂,这主要是由于稳定同位素13C的掺入,致使C60分子结构的对称性降低.这种稳定同位素13C标记富勒烯13C-C60,将为安全、无损、定量的富勒烯纳米材料体内的生物安全性研究奠定重要基础.更多还原

【Abstract】 In the last three decades, due to the unique structure and biological activities of fullerene materials, many reports were published on their synthesis and application in biomedical and environmental sciences, however, there is very few examples that have leapt out of the laboratory and into the commercial market. Obviously, further study is required to solve their crucial biological safety issues. In this article, the 13C-C60 sample was synthesized by arc discharge method with the stable isotope 13C direct substituting the skeleton carbon atoms on the carbon cage. The numbers of the incorporated 13C-atoms have been identified by isotope ratio mass spectroscopy. The isotope effect of the skeleton 13C-enriched C60 made its mass spectrum and vibrational Infrared/Raman spectra changes. Its mass spectra peaks at range from m/z=719~734 showed the normal distribution and the strongest peak position shifted up with the increase number of 13C. Infrared and Raman spectra of 13C-C60 retained the characteristic spectrum of C60, however, due to the stable isotope 13C-incorporated reducing C60 molecular symmetry, the characteristic peaks occurred the migration and splitting with increasing the number of 13C. Simultaneously, the 13C direct labeling method does not destroy the intrinsic structure of fullerene. Undoubtedly, 13C-C60 will open the door for quantitatively, safely and non-destructively evaluating on the biological safety of fullerene nanomaterials in vivo.更多还原