【摘要】 目的:研究Runt相关转录因子3(RUNX3)、RAS相关区域家族1A(RASSF1)在口腔黏膜下纤维性变(OSF)及其癌变组织中的表达与分布,初步探讨二者在OSF癌变过程中的作用。方法:采用免疫组织化学SP法检测10例正常口腔黏膜组织、35例OSF组织、10例OSF癌变组织中RUNX3、RASSF1A蛋白的表达及分布。结果:RUNX3在正常口腔黏膜组阳性表达率为90.00%(9/10),OSF组为85.71%(30/35),OSF癌变组为30.00%(3/10),正常对照组与OSF组无统计学差异,OSF癌变组显著低于正常对照组(P<0.05)及OSF组(P<0.05)。RASSF1A在正常口腔黏膜组阳性表达率为80.00%(8/10),OSF组为62.86%(22/35),OSF癌变组为20.00%(2/10),正常对照组与OSF组无统计学差异,OSF癌变组织组显著低于正常对照组(P<0.05)及OSF组(P<0.05)。结论:RUNX3和RASSF1A表达下调或缺失与OSF癌变有关。更多还原

【Abstract】 Objective:To investigate the expression of RUNX3 and RASSF1Aprotein in oral submucous fibrosis(OSF)and OSF carcinogenesis tissues and to evaluate their possible roles in the carcinogenesis of OSF.Methods:Immunohistochemical staining by SP method was used to detect the expression of RUNX3 and RASSF1Ain 10 cases of normal oral mucosa tissue,35 cases of OSF and 10 cases of OSF carcinogenesis tissues.Results:The positive expression rate of RUNX3 in normal oral mucosa epithelium was 90%(9/10),OSF group was 85.71%(30/35)and OSF carcinogenesis group was 30%(3/10).The positive expression rate of normal control group and OSF group had no statistical difference,but OSF carcinogenesis group was significantly lower than the control group(P<0.05)as well as OSF group(P<0.05).The positive expression rate of RASSF1 Ain the normal oral mucosa epithelium was 80.00%(8/10),OSF group was 62.86%(22/35)and OSF carcinogenesis group was 20%(2/10).The positive expression rate of normal control group and OSF group had no statistical difference,while OSF carcinogenesis group was significantly lower than the control group(P<0.05)as well as OSF group(P<0.05).Conclusion:The down-regulation or loss of expression of RUNX3 and RASSF1Amay relate to OSF carcinogenesis.更多还原