【摘要】 【目的】刺桐姬小蜂Quadrastichus erythrinae Kim体型小,传统的形态学鉴定方法难以快速准确识别。【方法】本研究测定了刺桐姬小蜂的rDNA ITS1和ITS2序列,根据18S rDNA部分序列,利用MEGA的最大相似法(Maximum Likehood)构建系统发育树。根据刺桐姬小蜂ITS1和ITS2序列设计了特异引物,应用特异引物对单只刺桐姬小蜂进行PCR扩增,可稳定地扩增出明显的目的DNA条带。【结果】研究表明,基于ITS基因的DNA条形码技术可以用于刺桐姬小蜂的快速准确鉴定。【结论】因此,采用ITS1和ITS2区的特异性引物可对刺桐姬小蜂进行快速分子鉴定。更多还原

【Abstract】 [Objectives]Quadrastichus erythrinae Kim(Hymenoptera:Eulophidae) is too small to be identified quickly and accurately by traditional morphological methods.[Methods]The complete sequences of the rDNA ITS1 and ITS2 genes were determined for Q.erythrinae.A phylogenetic tree was established on the basis of variation in 18 SrDNA part sequences using MEGA Maximum Likelihood.Species-specific primers of Q.erythrinae were designed based on the rDNA ITS1 and ITS2 sequences.The results of PCR amplification of rDNA ITS1 and ITS2 indicate that these species-specific primers could reliably identify this species.[Results]The results suggest that the species-specific primers developed produce clear,unique and reproducible target DNA bands from individual adult wasps.Molecular identification of Q.erythrinae using species-specific primers from the rDNA ITS1 and ITS2 region is therefore feasible.[Conclusion]This study identified a possible avenue for the molecular identification of Q.erythrinae.更多还原