【摘要】 目的:研制一种新型的碳磷灰石骨修复材料,初步观察其成骨作用。方法:以鸡蛋壳为原料,采用微波法制备不同加热条件下的骨修复材料,利用扫描电镜(SEM)、X射线衍射仪(XRD)、傅立叶红外(FT-IR)和能谱分析(EDXA)手段,表征微波处理前后材料的理化性能。进一步选用新西兰兔制备直径5mm的颅骨全层缺损区,以未经微波处理的鸡蛋壳粉及Bio-Oss作为对照,充填经微波处理后的新型骨修复材料,8周后观察新生骨的生成情况。结果:SEM,XRD,FT-IR和EDXA分析证实:随加热时间延长,材料的微观形态由针状向球状转变,碳磷灰石的特征性波峰也不断增强;研制的新材料含有与人体骨相似的碳、磷和钙成分。动物实验的结果表明,经微波处理的碳磷灰石鸡蛋壳粉充填的缺损区,比未处理组和空白对照组的缺损区有更多的矿化新生骨和较少的未矿化新生骨,接近Bio-Oss的效果。结论:微波加热法能有效地将天然碳酸钙转化为碳磷灰石,该材料有望作为一种潜在的新型骨修复材料。更多还原

【Abstract】 Objective To develop a new bone substitute derived from avian eggshell(ES) using microwave processing and observe its abilities of bone formation. Methods Calcium carbonate ES powder was prepared by microwave method with different heating time. The physicochemical characteristics of the new materials with or without microwave heating were analyzed by SEM、EDXA、XRD、FT-IR. A pilot animal study was conducted by creating standard defects of 5 mm diameter on parietal bone of New Zealand rabbits. The defects were filled with 60 min microwave heated ES powder whereas using microwave untreated ES powder and Bio-Oss in comparison. After 8 weeks new bone formation of defects were observed. Results SEM, XRD, FT-IR and EDXA analysis showed the micromorphology of the new materials turned from needle-like to ball-like structures as the microwave heating time prolonged. The characteristic peak of calcium carbonate was also increased at the same time. Carbon, phosphorus and calcium were confirmed in the new material as well. Animal study showed 5 mm defects on parietal bone filled with calcium carbonate ES powder had more calcified bone formation and less uncalcified new bone. Conclusions The study demonstrated the efficient use of microwave in converting ES powder from natural CaCO3 into calcium carbonate. The new material might be a potential bone substitute.更多还原