【摘要】 目的研究杜仲叶总提取物对去势大鼠骨质疏松(osteoporosis,OP)模型的骨代谢调节作用机制。方法 4月龄雌性SD大鼠32只,随机分成正常对照组(Control组,生理盐水灌胃)、去卵巢对照组(OP组,生理盐水灌胃)、雌二醇组(Estrogen组,雌二醇水溶液灌胃)、杜仲叶总提取物组(DZ组,杜仲叶总提物水溶液灌胃)。各组治疗3个月后,通过眼眶采血,测定血清中Ca2+、P3+和ALPase活力。并取大鼠的股骨和胫骨,用双能X线骨密度仪测定骨密度。结果血清中Ca2+、P3+和ALPase活力,DZ组与OP组比较,均有显著差异(P<0.05);股骨和胫骨BMD,DZ组与OP组比较,有显著差异(P<0.05)。结论杜仲叶总提取物可以改善OP模型动物骨代谢,增加骨质疏松模型动物的骨密度,减少骨破坏,加强骨稳定,有效防治骨质疏松症。更多还原

【Abstract】 Objective Study of adjusting effect of total extraction in Eucommia ulmoides oliv leaf on bone metabolism in osteoporosis(OP) model animal’s OP and its mechanism. Methods Thirty-two SD female rats at the age of 4 months were randomly divided into 4 groups: normal control group(gavage with physiological saline),ovariectomized group(OP group,gavage with physiological saline),estrogen group(gavage with estrogen),total extraction in Eucommia ulmoides oliv. Leaf group(DZ group,gavage with total extraction of Eucommia ulmoides oliv. leaf). At the end of 3-month treatment,blood calcium(Ca2+),blood phosphor(P3+),and alkaline phosphates(ALP) were tested by orbital blood collection. Thon femur and tibia of 4-group animals were taken out.Bone mineral density(BMD) of them was determined using dual-energy X-ray absorptiometry. Results Compared with OP group,blood calcium(Ca2+),blood phosphor(P3+),and alkaline phosphatase(ALP) in DZ group were decreased significantly(P<0.05);bone mineral density of femurs and tibia in DZ group were remarkably increased(P<0.05). Conclusion Total extraction in Eucommia ulmoides oliv. leaf can improve bone metabolism of OP animals,increase BMD,prevent and treat osteoporosis effectively.更多还原