【摘要】 目的探讨脐带(UC)间充质干细胞(MSC)生物学的特性。方法行剖宫产术时采集胎儿UC,采用1g·L-1Ⅱ型胶原酶消化、分离UC-MSC。将第3代UC-MSC随机分为3组:正常对照组、诱导向脂肪细胞分化组和诱导向软骨细胞分化组,分别在光学显微镜下观察UC-MSC形态,流式细胞仪检测UC-MSC表面标志,体外诱导UC-MSC分化为软骨及脂肪细胞。结果第3代UC-MSC经流式细胞仪测定证明,其高表达CD29、CD105、CD166和CD90,不表达CD34、CD45。正常对照组的UC-MSC细胞呈长梭形,排列有明显方向性;诱导向脂肪细胞分化组的UC-MSC细胞呈圆形或近似圆形;诱导向软骨细胞分化组的UC-MSC细胞呈梭形,也有呈圆形或近似圆形。油红O染色证明UC-MSC经诱导分化成脂肪细胞,阿新兰染色UC-MSC经诱导分化成软骨细胞。结论UC-MSC具有多能性,其可作为一种新的MSC来源,并可用于临床干细胞移植。更多还原

【Abstract】 Objective To study the biological characteristics of mesenchymal stem cells derived from umbilical cord(UC-MSCs).Methods The umbilical cords were obtained from pregnancies delivered by caesarean section.UC-MSCs were isolated by typeⅡcollagenase digestion.The UCMSCs at the third generation were randomly divided into three groups:normal control group,differentiation into adipocyte group and differentiation into chondrocyte group.The morphology of UC-MSCs was observed under an optical microscope.The surface markers of UC-MSCs were detected by flow cytometry.In addition,the differentiation of UC-MSCs into adipocytes and chondrocytes were observed.Results Flow cytometry showed that UC-MSCs highly expressed CD29,CD105,CD166and CD90but did not express CD34and CD45.UC-MSCs showed spindle shape and arranged with obvious directivity.Cells differentiated toward adipocytes showed round or approximately round shape.Cells differentiated toward chondrocytes showed spindle,round or approximately round shape.Moreover,cells differentiated into adipocytes and chondrocytes were proved by oil red O staining and alcian blue staining,respectively.Conclusion UC-MSCs are pluripotent cells that may be used as a new source for stem cell transplantation.更多还原