【摘要】 采用自溶-超声波耦合法提取啤酒废酵母中β-1,3-D-葡聚糖。首先以自溶率为考察指标,将单因素和正交实验相结合,结果显示,pH=5.0条件下,质量分数5%NaCl和体积分数1.5%乙酸乙酯混合添加可以最大限度地促进酵母细胞的自溶,从而促进酵母细胞质壁分离。进而,采用超声波法从自溶后收集的细胞壁沉淀中提取β-1,3-D-葡聚糖,以质量分数2%NaOH作超声溶剂,利用Box-Behnken设计考察了超声功率、超声时间和超声次数对葡聚糖提取率的影响,确定最佳提取条件为:超声功率550 W,超声时间33 min,超声次数2次,在该条件下葡聚糖提取率达到50.5%,与纯超声波提取葡聚糖法相比提取率提高了2倍。更多还原

【Abstract】 Autolysis-ultrasonic coupling method was adopted to extract β-1,3-D-glucan from waste beer yeast. Firstly,single factor and orthogonal test were designed to study the effect of different autolysis promoters on yeast autolysis. The results show that the mixture of 5%( mass fraction) NaCl + 1. 5%( volume fraction) ethyl acetate can effectively promote yeast autolysis. Furthermore,ultrasonic method was used to extract β-1,3-D-glucan from the cell wall deposition harvested after autolysis. The effects of ultrasonic power,ultrasonic time and ultrasonic times were explored in 2%( mass fraction) NaOH solvent by response surface methodology. The results indicate that the optimum parameters were ultrasonic power 550 W,ultrasonic time 33 minutes,and 2 ultrasonic times. Under the above conditions,the extraction efficiency of β-1,3-glucan was up to 50. 5%,which was doubled as compared with pure ultrasonic method without autolysis pretreatment.更多还原