【摘要】 目的:检测食管鳞癌患者不同临床分期癌组织及癌旁组织中MicroRNA let-7e表达水平的差异。方法:应用实时荧光定量RT-PCR Taqman MGB探针法,检测60例食管癌癌组织及癌旁组织中MicroRNA let-7e表达水平。以U6 snRNA为内参,进行荧光定量PCR,统计分析表达水平的差异。结果:MicroRNA let-7e的表达水平在癌组织比癌旁组织高,病理分期I期与III期相比,差异有统计学意义(P<0.05)。结论:MicroRNA let-7e在食管鳞癌中表达水平明显高于癌旁组织,可能用于食管鳞癌临床分期诊断的重要标记。更多还原

【Abstract】 Purpose: To investigate the expression levels of MicroRNA let-7e in esophageal squamous cell cancer patients and to compare cancer tissues with adjacent normal lung tissues in different clinical stages. Method: The MicroRNA let-7e expression levels in cancer tissues and adjacent normal lung tissues were detected by quantitative real-time reverse transcription-PCR from 60 esophageal squamous cell cancer patients who were divided into three groups, with U6RNA as an actin. Results: The expression levels of MicroRNA let-7e in cancer tissues were significantly higher than in adjacent normal esophageal tissues, and had statistical significance in different stages of the tumors: characteristics, lymph metastasis, differentiation, and the stage of the tumors. Conclusions: The study suggested that microRNA let-7e was significantly up-expressed in esophageal squmaos cell cancer and should be a useful biomarker for the diagnosis of clinical stages of esophageal cancer patients.更多还原