【摘要】 目的探讨地域差异对人体血清代谢谱的影响。方法采集来自我国9个省份的血清样本,共计169例,按照地理位置的归属,将样本划分为东西南北4个区域。采用核磁共振(NMR)的代谢组学技术与多元统计方法相结合,对血清NMR谱图进行分段积分、归一化处理,所得数据输入到多元统计分析软件SIMCA-P 10.0,经正交信号校正(OSC)后采用偏最小二乘法(PLS)进行模式识别,寻找东西部人群、南北方人群血清中具有差异的代谢物。结果东西部、南北方健康人群的血清代谢谱中,葡萄糖、脂蛋白、氨基酸、酮体、肌酸、胆碱等多种小分子代谢产物浓度存在差异。结论由多种因素(气温、降水、民俗文化、生活习惯等)造成的地域差异会导致人体血清代谢谱的差异,可以对流行病学及营养代谢组学的研究提供相应的参考。更多还原

【Abstract】 Objective To investigate the effect of geographical differences on human serum metabolic profiles. Methods A total of 169 serum samples were collected from healthy individuals from 9 provinces in China and divided into four groups according to the geographical location. The nuclear magnetic resonance(NMR) data of these samples were segmentally integrated,normalized,and analyzed with multivariate statistical methods using SIMCA-P 10. 0 software to investigate the difference in serum metabolites between eastern and western populations or between northern and southern populations. Results There were differences in a variety of small molecule metabolites in serum between eastern and western populations or between northern and southern,including glucose,lipoproteins,amino acids,ketones,creatine and choline. Conclusion The geographical differences in a variety of factors( temperature,precipitation,folk culture,living habits,etc) result in the differences in human serum metabolic profiles,which can provide appropriate reference for epidemiological studies and nutritional metabonomics.更多还原