As common food,egg with its cholesterol content may affect human health. The results showed that natural active substances could reduce significantly the concentration of egg cholesterol,such as flavonoids,phytosterols,polysaccharide,other plant and plant extracts and so on. It proved to be the very effective nutrition regulator for laying hens. In this study,the application effect of egg cholesterol regulated and controlled by the additive of natural active substances was introduced.