【摘要】 目的评价辛伐他汀对兔股动脉球囊损伤后血管狭窄和平滑肌细胞增殖的影响。方法将24只雄性新西兰兔随机分为假手术组、动脉狭窄模型组和辛伐他汀组,每组8只。除假手术组外,其余两组行股动脉球囊损伤,辛伐他汀组给予辛伐他汀灌胃干预。术后第14天处死实验兔,取出血管标本,处理后行HE染色观察组织病理变化。计算机病理图像分析系统测量动脉管腔、内弹力层、外弹力层围绕面积,计算内膜、中膜面积和内膜/中膜面积比值,免疫组化法检测动脉壁增殖细胞核抗原(PCNA)表达,观察平滑肌细胞增殖情况。结果与动脉狭窄模型组相比,辛伐他汀组血管管腔面积增加,但差异无统计学意义(P=0.057),新生内膜面积显著减少(P=0.006),内膜/中膜面积比值降低,但差异无统计学意义(P=0.16),血管壁PCNA的阳性表达指数显著降低(P=0.003)。结论辛伐他汀可以显著抑制兔股动脉球囊损伤后内膜增生和平滑肌细胞增殖。更多还原

【Abstract】 Objective To evaluate the effects of simvastatin on vascular stenosis and vascular smooth muscle cell(VSMC) proliferation of balloon-injured femoral artery in experimental rabbits. Methods A total of 24 male New Zealand white rabbits were randomly and equally divided into three groups: the sham group(n = 8), the artery stenosis model group(n = 8) and the simvastatin group(n = 8). Femoral artery stenosis was induced by injuries with balloon in both the model group and the simvastatin group, except for the sham group. The rabbits in simvastatin group were fed with simvastatin. The rabbits were sacrificed on the 14th day after operation, and the femoral arteries were removed and sent for the pathological examination. The internal elastic lamina, external elastic lamina and luminal areas were measured. Then the areas of intima, media and the intima-to-media ratio were calculated. In order to observe the proliferation of VSMC, proliferating cell nuclear antigen(PCNA) expression of arterial wall was tested and observed by immunohistochemistry method.Results In comparison with the model group, the average luminal area of the simvastatin group was increased although the difference was not statistically significant(P = 0.057). The area of intima of simvastatin group was decreased(P = 0.006), and the intima-to-media ratio was notably decreased, but the difference was of no statistic significance(P = 0.16). The PCNA expression of arterial wall was remarkably decreased(P = 0.003). Conclusion Simvastatin can effectively inhibit the intimal hyperplasia and the vascular smooth muscle cell proliferation of balloon-injured femoral artery in experimental rabbits.更多还原