【摘要】 目的:研究丹参酮ⅡA对坐骨神经慢性压迫大鼠的的镇痛效果及大鼠脊髓背角内半胱氨酸天冬氨酸蛋白酶3(caspase-3)和胶质纤维酸性蛋白(GFAP)表达的影响。方法:检测大鼠在手术前及术后14 d的机械痛阈和热痛阈;PCR及免疫组织化学分别检测大鼠脊髓背角内caspase-3和GFAP基因及蛋白的表达,TUNEL法检测脊髓背角内的细胞凋亡。结果:与假手术组比较,模型组大鼠的机械痛阈和热痛阈明显降低,caspase-3和GFAP的表达均增多,凋亡染色阳性细胞数目也增多;与模型组比较,丹参酮ⅡA处理组大鼠的机械痛阈和热痛阈明显升高,脊髓背角内caspase-3和GFAP的表达均下降,凋亡染色阳性细胞数目也减少,差异均有统计学意义。结论:鞘内注射丹参酮ⅡA对坐骨神经慢性压迫模型大鼠有镇痛作用,其机制可能与降低脊髓背角内caspase-3和GFAP的表达有关。更多还原

【Abstract】 Objective:To study the effect of tanshinone TJA on neuropathic pain and the change of expression of caspase-3 and GFAP on rat Methods:Before and after operation,mechanical nociceptive thresholds were assessed with paw withdrawal thresholds（PWTs） to von Frey filament and the paw withdrawal thermal latency（PWTL） was examined Caspase-3 and GFAP were measured with RT-PCR and immunohistochemical staining and apoptosis was tested by TUNEL method in the spinal dorsal horn of groups on the 14^th day after operation.Results:In the model group,the PWTs and PWTL decreased,the expression of caspase-3 and GFAP increased,and the apoptotic cells also increased significantly in the spinal dorsal horn compared with the sham group 14 days after operation.In the tanshinone ⅡA group,the PWTs and PWTL increased,the expression of caspase-3and GFAP decreased,and the apoptotic cells also decreased significantly in the spinal dorsal horn compared with the model group14 days after operation.Conclusion:Intrathecal injection of tanshinone ⅡA has an analgesic effect on neuropathic pain of rats,which may be through decreasing the expression of caspase-3 and GFAP in the spinal dorsal horn.更多还原