【摘要】 目的:观察米诺环素干预对脊髓横断大鼠行为功能和横断部位胶质纤维酸性蛋白(GFAP)表达的影响。方法:雄性Wistar大鼠随机分为3组,假手术组、模型组和米诺环素预处理组。米诺环素预处理组大鼠术前腹腔注射米诺环素(90mg/kg)。各组大鼠于术后1、7、14、21 d应用Molt斜板试验和BBB评分方法对大鼠后肢行为功能进行评价。第22天将所有实验大鼠处死后取损伤段脊髓,H-E染色观察其病理变化、免疫组织化学检测GFAP的表达。结果:术后第1天模型组和米诺环素预处理组大鼠Molt斜板障碍率无明显差别,BBB运动功能评分也无明显差别;而米诺环素预处理组术后第7天到第21天Molt斜板障碍率较模型组有明显下降,且BBB运动功能评分明显高于模型组。H-E染色显示,模型组大鼠有明显的病理变化,而米诺环素预处理组可改善损伤脊髓的病理形态。免疫组织化学显色显示,与模型组相比,米诺环素预处理组GFAP阳性细胞数减少,差异具有统计学意义。结论:米诺环素预处理可有效抑制脊髓横断大鼠神经胶质细胞的过度增殖,有利于神经功能的恢复。更多还原

【Abstract】 Objective:To observe the effects of minocycline pretreatment on the behavioral function and the expressions of glial fibrillary acidic protein(GFAP) in rats with completely transected spinal cord.Methods:Adult male Wistar rats were randomly divided into a sham operation group,a transected spinal cord injury model group(tSCI) and a minocycline pretreated group.The rats in the minocycline pretreated group received minocycline intraperitoneal injection before operation(90 mg/kg).The functional tests were performed at day 1,7,14 and 21 including ratio of Molt’s inclined plane test and Basso-Beattie-Bresnahan(BBB) locomotor rating scale on all rats.All injured rats were sacrificed on the 22 day and the injured spinal cords were taken out for morphological studies by HE staining.The changes of GFAP expression at spinal cord injury site were detected by using immunohistochemical method.Results:One day after injury,there was no significant difference in the ratio of Molt’s inclined plane scales and BBB locomotor rating scale between the tSCI model group and the minocycline pretreated group.But the ratio of Molt’s inclined plane scales was descended significantly and the BBB score was increased significantly from 7 days to 21 days after injury in the minocycline pretreated group as compared with that of the tSCI model group.There appeared obvious pathological changes of tSCI rats in the model group,but pathological morphology of spinal cord was significantly improved in minocycline pretreated group by HE staining.The number of GFAP positive cells in the spinal cord significantly increased after SCI but significantly decreased in the minocycline pretreated group.Conclusion:Minocycline pretreatment can effectively inhibit the excessive proliferation of neuroglial cells in rats with transected spinal cord injury and promote the recovery of nerve function.更多还原