【摘要】 目的:原位检测神经干细胞/祖细胞在成年小鼠室周器官分布。方法:成年BALB/c雄性小鼠,7~8周龄,连续腹腔注射BrdU(50 mg/kg)3 d,4 d后取脑组织,连续冷冻切片,用免疫荧光显色检测Nestin/SOX2及SOX2/BrdU在成年小鼠室周器官细胞的双表达。结果:在成年小鼠正中隆起(ME)、穹隆下器(SFO)及最后区(AP)均可见大量SOX2、中量巢蛋白及少量BrdU阳性细胞,并可见一些Nestin/SOX2及SOX2/BrdU双阳性细胞。成年小鼠室周器官Nestin/SOX2及SOX2/BrdU与脑室下区Nestin/SOX2及SOX2/BrdU双阳性细胞比较具有差异。结论:成年小鼠室周器官存在有一定数量的Nestin/SOX2及SOX2/BrdU双阳性细胞,这表明成年小鼠室周器官可能存在具有增殖和分化潜能的神经干细胞/祖细胞。更多还原

【Abstract】 Objective:To study whether there exists neural stem/progenitor cells which can proliferate in the adult mouse circumventriculer organ(CVOs).Methods:The adult health BALB/c mice were injected BrdU(50 mg/kg) by abdominal cavity for three days,the mice were perfused using 4%paraformaldehyde phosphate buffered saline after four days,and then the brain was processed for tissue sectioning and immunofluorescence staining for Nestin/SOX2 and SOX2/BrdU double positive cells.Results:A large number of SOX2,modest number of Nestin and small number of BrdU positive cells can be found in median eminence(ME),subfornical organ(SFO),area postrema(AP).And some Nestin/SOX2 and SOX2/BrdU double positive cells were also found in these parts.The percentages of Nestin/SOX2 and SOX2/BrdU double postive cells were observably different between CVOs and subventricular zone.Conclusion:A certain amount of Nestin/SOX2 and SOX2/BrdU double positive cells were found in CVOs of adult mice,suggesting the existence of neural stem/progenitor cells,which can proliferate and differentiate.更多还原