【摘要】 目的:研究异丙肾上腺素致大鼠心肌肥厚中磷酸化磷脂酞肌醇3-激酶(p-PI3K)和磷酸化蛋白激酶B(p-Akt)表达与心肌纤维化的关系,为探讨大鼠心肌肥厚中心肌纤维化的信号转导机制和改善心功能提供形态学资料。方法:健康成年SD大鼠皮下注射异丙肾上腺素,造成心肌肥厚模型;取心肌组织,常规石蜡切片,Masson染色,观察心肌组织的成纤维细胞变化;免疫组织化学显色和免疫荧光显色,检测p-PI3K和p-Akt的表达及分布。利用图像分析软件对p-PI3K和p-Akt的表达结果进行定量分析。结果:大鼠实验组的心肌肥厚指标明显高于对照组;Masson染色结果显示实验组心肌细胞较对照组心肌细胞直径明显变大,细胞间距变大,细胞间纤维细胞增多,呈现区域性纤维化;免疫组织化学检测显示,实验组心肌组织p-PI3K和p-Akt蛋白表达面积和平均光密度较对照组高,两者差异有统计学意义;免疫荧光检测实验组心肌组织p-PI3K和p-Akt蛋白表达较对照组高。结论:p-PI3K和p-Akt蛋白表达增高可能在心肌纤维化的发生和发展过程中起重要作用。更多还原

【Abstract】 Objective:To provide morphological data for the investigation of the cardiac hypertrophy signal transduction mechanism and reversal of cardiac hypertrophy by investigating the expression of p-PI3 K and p-Akt in cardiac tissues in isoproterenol-induced myocardial hypertrophy.Methods:On the observation of the expression of p-PI3 K and p-Akt in cardiac tissue in isoproterenol-induced myocardial hypertrophy,the morphological data was provided for the investigation of the cardiac hypertrophy signal transduction mechanism and reversal of cardiac hypertrophy.Cardiac hypertrophy models were established by subcutaneous injection of isoproterenol on healthy adult SD rats.The pathological changes of the myocardial tissues with conventional paraffin section and Masson staining were observed.The expression and distribution of p-PI3 K and p-Akt was detected by immunohistochemistry and immunofluorescence staining.Image analysis software was used to conduct quantitative analysis.Results:Compared with the control group,the positive expression of p-PI3 K and p-Akt in the experimental group was significantly increased.Conclusion:The increase of p-PI3 K and p-Akt protein expression may play an important role in the occurrence and development of cardiac hypertrophy.更多还原