【摘要】 目的:通过检测胎膜早破孕妇母血、胎膜组织中MMP-9、IL-6的表达水平,探讨其与胎膜早破、亚临床绒毛膜羊膜炎的相关性,探索预测和诊断亚临床绒毛膜羊膜炎的可行性的生物学指标。方法:ELISA方法检测胎膜早破及对照组孕妇血清MMP-9、IL-6的水平,同时取胎膜破口边缘处胎膜,采用免疫组化法(SP法)检测MMP-9、IL-6在胎膜上的表达;且病理检查确定有无组织学绒毛膜羊膜炎。结果:①胎膜早破孕妇血清MMP-9、IL-6水平明显高于正常对照组;②胎膜早破组胎膜中MMP-9、IL-6表达明显高于正常对照组;③母体血清MMP-9浓度为82.97 pg/ml时,IL-6浓度为46.38 pg/ml时,可作为预测临床绒毛膜羊膜炎的阈值。结论:MMP-9、IL-6可作为预测胎膜早破、合并亚临床绒毛膜羊膜炎的可行性生物学指标,联合检测更有利于早期诊断和预测。更多还原

【Abstract】 Objective Through detect the expression of MMP9,IL6 in maternal serum and fetal membrane in pregnant woman with premature rupture of membranes,to approach the relationship between them,premature rupture of membranes and subclinical chorioamnionitism,the feasibility of biological indicators to anticipate and diagnose subclinical chorioamnionitis. Methods Women with premature rupture of membranes and normal women were studied,expression of MMP9,IL6 in maternal serum were measured by enzymelinkedimmunosorbent assay. At the same time,the expression of MMP9,IL6 in fetal membranes,which collected from the edge of fetal membranes rupture membranes,were detected by immunohistochemical method( SP). Chorioamnionitis were diagnosed by histopathologically Results ① The lever of MMP9,IL6 in maternal serum in pregnant woman with premature rupture were significantly higher than that in the control group. ②The lever of MMP9,IL6 in fetal membranes were significantly higher in pregnant woman with premature rupture of membranes than in women in controls. ③Maternal serum MMP9 lever 82. 97 pg / ml ng / ml,IL6 lever 46. 38 pg / ml ng / ml were regarded as the diagnosis threshold value of the subclinical chorioamnionitis. Conclusion serum MMP9,IL6 lever is a useful biological indicators for early detection of premature rupture of membranes and subclinical chorioamnionitis. And Joint detection is more conducive to early diagnosis and prediction.更多还原