【摘要】 目的研究miR-196a-5p在小鼠胚胎干细胞(m ESC)自我更新中的功能。方法定量PCR法确定miR-196a-5p在m ESC早期分化过程中的表达。在胚胎干细胞中过表达miR-196a-5p类似物,通过集落形成实验、细胞周期分析、碱性磷酸酶染色和Oct4染色确定miR-196a-5p过表达对m ESC自我更新及增殖的影响。结果 miR-196a-5p在m ESC早期分化过程中表达上升(P<0.05),在m ESC中过表达miR-196a-5p类似物后,ESC的集落形成及增殖能力被明显抑制(P<0.01),碱性磷酸酶活性下降,Oct4蛋白水平也出现明显下降。结论 miR-196a-5p具有抑制m ESC自我更新的能力,在m ESC早期分化过程中可能发挥重要作用。更多还原

【Abstract】 Objective To study the role of miR-196a-5p in regulating self-renewal of mouse embryonic stem cells( m ESC). Methods Q-PCR was used to detect the expression of miR-196a-5p during m ESC early differentiation.miR-196a-5p mimics were overexpressed in m ESCs,and the then colony formation ability,cell proliferation,Alkaline Phosphatase( AP) activity and Oct4 staining of m ESC were detected. Results miR-196a-5p increased during RA-induced differentiation and EB differentiation of m ESC. Overexpression of miR-196a-5p in m ESC could inhibit the colony formation and cell proliferation of m ESCs. Meanwhile,miR-196a-5p decreased the AP activity and Oct4 expression in m ESCs. Conclusions miR-196a-5p suppresses self-renewal of m ESCs and increases during m ESC early differentiation which suggests that miR-196a-5p might play important roles in promoting m ESC differentiation.更多还原