【摘要】 目的观察PPAR-γ配体吡格列酮(PGZ)调节绒癌细胞系JEG-3的增殖、分泌h CG和体外侵袭、转移能力。方法用MTT法和IEMA测定细胞的增殖和分泌h CG。Matrigel侵袭模型分析细胞的迁徙和侵袭能力。结果小剂量PGZ对JEG-3有增殖抑制作用,随PGZ浓度的增加,JEG-3透过Matrigel膜的细胞数明显减少(P<0.01)。结论PGZ明显抑制JEG-3肿瘤细胞的生长和侵袭能力,提示PPAR-γ途径可能是妊娠滋养细胞肿瘤治疗的新方向。更多还原

【Abstract】 Objective To observe the effect of ligand of peroxisome proliferators-activated receptor gamma( PPAR gamma) on the proliferation and secretion of trophoblast and choriocarnoma cells( JEG-3) and to study the role played by PPAR gamma during the process of JEG-3 activation. Methods We employed MTT assay to observ the growth of the cells and IEMA the secretion of h CG in the cells. Matrigel invision chambers were performed to analyse the cell migration and invasion. Results The proliferation of JEG-3 was suppressed by PGZ with a low dosedependent manner. The numbers of JEG-3 was obviously inhibited by PGZ( P < 0. 01) with increasing concentration. Conclusions The results obtained with this in vitro system demonstrated that PPAR gamma activation may inhibit JEG-3 proliferation and invasiveness. PPAR gamma may be a new therapeutic target for gestational trophoblastic tumor in humans.更多还原