【摘要】 目的探讨转录激活因子4(ATF4)在小鼠动情周期中子宫内膜的表达规律。方法采用RT-PCR、免疫组织化学和Western blot法分别检测小鼠子宫内膜中ATF4 mRNA和蛋白在动情前期、动情期、动情间期和动情后期的表达规律。结果 ATF4 mRNA在动情期表达显著高于其他3期(P<0.05);ATF4蛋白主要在腺上皮细胞和腔上皮细胞胞质表达,其表达规律与RT-PCR的结果基本一致。结论 ATF4在小鼠动情周期子宫内膜中呈动态表达,提示ATF4可能参与了动情周期子宫内膜变化的调控。更多还原

【Abstract】 Objective To investigate the expression pattern of ATF4 in mouse endometrium during the estrus cycle. Methods Reverse transcription polymerase chain reaction(RT-PCR),immunohistochemistry and Western blot-ting were used to detect the expression of ATF4 mRNA and protein in mouse endometrium during at each stage of the estrus cycle. Results ATF4 mRNA expression in estrus stage was higher than those of the other 3 stages,and there was significant difference between estrus stage and the other 3 groups(P < 0. 05). The prominent expression of ATF4 protein was primarily limited to the glandular epithelium cell and luminal epithelium cell cytoplasm in the endometrium. Expression of ATF4 protein was similar with that of the mRNA. Conclusions ATF4,expressed in en-dometrium periodically in the estrus cycle,might play an important role in endometrial cyclical variation during the estrus cycle.更多还原