【摘要】 目的分析宫颈癌移植瘤小鼠的癌与癌旁组织中miR-15b和miR-16的表达状况以及NF-κB信号通路在宫颈癌癌旁组织活化情况,为宫颈癌的诊断和治疗提供新思路。方法建立小鼠宫颈癌移植瘤模型,取癌组织与癌旁组织,以及正常的脂肪组织,用实时荧光定量PCR方法检测miR-15b和miR-16的表达,用免疫组化检测NF-κB(P65)蛋白的表达及定位。结果移植瘤造模成功。miR-15b在宫颈癌组织中表达为32.21±3.67,明显高于癌旁组织的25.16±1.86和正常组织的1.00±0.12(P<0.05),癌旁组织也显著高于正常组织(P<0.05);miR-16在宫颈癌组织中表达为28.63±2.34,明显高于癌旁组织的22.16±1.76和正常组织的1.00±0.12(P<0.05),癌旁组织也显著高于正常组织(P<0.05)。在正常脂肪组织中,NF-κB(P65)蛋白主要定位于胞质,而在癌组织与癌旁组织中,NF-κB(P65)蛋白主要位于胞核。结论 NF-κB通路及其相关的miR-15b和miR-16可能参与了宫颈癌发生的早期分子事件。更多还原

【Abstract】 Objective To investigate the expression of miR-15b and miR-16 as well as NF-κB signaling in xeno-grafted cervical cancer and its adjacent tissues. Methods The xenograft mouse model of cervical cancer was suc-cessfully established. Xenografted cancer and its adjacent tissues,normal fat tissues were collected respectively. The expression of miR-15b and miR-16 was detected by real-time PCR. The expression and sublocation of NF-κB(P65)was analyzed by immunohistochemistry technology. Results HE staining of the xenograft indicated that the model was successful. The miR-15b level in the cancer were 32. 21 ± 3. 67,which were significant higher than that in adjacent tissues 25. 16 ± 1. 86 and normal fat tissue 1. 00 ± 0. 12(P <0. 05),miR-15b-level in adjacent tis-sues was also significantly higher than that in normal fat tissue(P <0. 05); miR-16 levels in the cancer were 28. 63 ± 2. 34,which was significantly higher than that in adjacent tissues 22. 16 ±1. 76 and in normal fat tissue 1. 00 ± 0. 12(P < 0. 05),miR-16 level in adjacent tissues was also significantly higher than that in normal fat tissue(P <0. 05). NF-κB(P65) was mainly found in cytosol of normal adipose tissues,whereas in cervical canc-er tissues and adjacent non-tumorous tissues,NF-κB( P65) translocated into the nucleus. Conclusions NF-κB signal pathway and its regulated miR-15b and miR-16 were significantly upregulated in both cancer tissues and adjacent tissues. The NF-κB signal pathway and its regulated miR-15b and miR-16 were involved in early molec-ular events of cervical cancer.更多还原