【摘要】 目的 研究电压一门控钠离子通道(VGSCs)亚型nNav1.5在人脑胶质瘤中的表达及其与肿瘤级别的关系。方法 用免疫荧光技术检测nNav1.5蛋白在胶质瘤U251细胞株中的表达定位;按2007年WHO胶质瘤分级,将胶质瘤标本分为低级别组(WHOⅠ~Ⅱ级,29例)和高级别组(WHOⅢ~Ⅳ级,37例),另外13例对照组织标本来自颅脑损伤内减压手术中切除的脑挫裂伤组织。采用RTPCR法、免疫组化法和Westem Blot法分别检测nNav1.5 mRNA和蛋白在胶质瘤组和对照组的表达情况。结果 nNav1.5主要在胶质瘤细胞核内表达,nNav1.5 mRNA和蛋白在胶质瘤组织和对照组织中均有表达,但其在胶质瘤中表达水平均显著升高(P<0.05),在高级别胶质瘤组的表达量亦高于低级别胶质瘤组,各组间比较差异有统计学意义(P<0.05)。结论 nNav1.5在人脑胶质瘤中表达上调并与肿瘤的恶性程度呈正相关,nNav1.5有可能是胶质瘤恶性增殖的一个调控因子,有望成为胶质瘤的一个新标记物和治疗的新靶点。更多还原

【Abstract】 Objective To investigate the expression of voltage-gated sodium channels(VGSCs)subunit nNav1.5 in human gliomas and its correlation to histopathological grades.Methods According to glioma grading criteria of WHO(2007),the gliomas from clinical samples were grouped as high grade(WHO,grade Ⅰ~Ⅳ,37 cases) and low grade(WHO,grade Ⅰ~Ⅱ,29 cases).And another 13 control cases were brain contusion tissue from brain injury decompression surgery resection.Immunofluorescence was used to localize the nNav1.5 protein in glioma U251 cell.Real-time PCR,immunohistochemistry and Western blotting were used to detect nNav1.5 mRNA and protein expression in gliomas and normal brain tissues from clinical samples,respectively.Results Immunofluorescent confocal microscope confirmed that nNav1.5 protein mainly localized in nuclei of U251 cells.The expression of nNav1.5 mRNA and protein was found in glioma groups and normal control group.Moreover,expression of nNav1.5 mRNA and protein in high grade group was statistically higher than in low grade group,and the differences were statistically significant among groups(P < 0.05 for each comparison).Conclusions nNav1.5 was over-expressed in gliomas and was directly correlated with tumor grades.nNav1.5 might be a regulatory factor for malignant proliferation of gliomas,therefore a potential biological marker and a new therapeutic target for gliomas.更多还原