【摘要】 目的了解2012年广西壮族自治区(广西)罗城县诺如病毒流行株基因型谱分布。方法 2012年收集323例5岁以下腹泻患儿粪便标本,反转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测诺如病毒,对VP1区部分核苷酸序列进行测定并构建核苷酸系统进化树。结果检测323例病例粪便标本,诺如病毒阳性24份(7.43%);诺如病毒GⅠ基因组2份(8.33%),GⅠ.4和GⅠ.6各1份;GⅡ基因组22份(91.67%),GⅡ.6基因型1份(1/22),GⅡ.4基因型21份(21/22)。GⅡ.4中,18份(85.71%)属于2006b变异株簇,3份(14.29%)属于Sydney变异株簇。结论 2012年广西罗城县诺如病毒流行株基因型呈现多样性,以GⅡ.42006b变异株为优势流行株,首次检出GⅡ.4 Sydney变异株。更多还原

【Abstract】 Objective To understand the genotypes of circulating Norovirus strains in Guangxi in 2012.Methods A total of 323 fecal samples collected from diarrheal children under 5 years old in 2012 were detected for Norovirus with RT-PCR,and corresponding sequences of partial VP1 region were analyzed and phylogenetic tree was constructed.Results Among the samples detected,twenty-four were Norovirus positive.Based on partial sequences of VP1 region,two strains belonged to genotype Ⅰ,twenty-two belonged to genotype Ⅱ.Three of 22 G II strains collected in November and December belonged to G Ⅱ.4 Sydney 2012 cluster /eighteen strains belonged to the 2006b cluster and one belonged to the G Ⅱ.6 cluster.Conclusion The circulating Norovirus genotypes were with diversity,and Norovirus GⅡ.4 Sydney variant was first detected in Guangxi.更多还原