【摘要】 目的研究二烯丙基二硫(DADS)是否通过下调组织蛋白酶D(Cathepsin D)的表达抑制人胃癌MGC803细胞侵袭转移。方法 RT-PCR和Western blot检测DADS及干扰Cath-D对人胃癌MGC803细胞Cath-D mRNA与蛋白表达变化;细胞划痕实验和Transwell实验分别检测DADS及干扰Cath-D对人胃癌MGC803细胞侵袭转移能力的变化。结果 DADS作用人胃癌MGC803细胞24 h后,Cath-D mRNA与蛋白表达水平明显下降(P<0.05),划痕距离明显增宽(P<0.05),穿膜细胞数明显减少(P<0.05);siRNA干扰Cath-D后,Cath-D mRNA与蛋白的表达水平明显下降(P<0.05),划痕距离明显增宽(P<0.05),穿膜细胞数明显减少(P<0.05)。结论 DADS能抑制人胃癌MGC803细胞的侵袭转移能力,其机制可能与下调Cath-D的表达有关。更多还原

【Abstract】 Objective To investigate whether diallyl disulfide( DADS) inhibits human gastric carcinoma MGC803 cells invasion and metastasis by down-regulating the expression of Cathepsin D. Methods The mRNA and protein expression level of Cath-D were detected by RT-PCR and Western blot respectively; Migration and invasion potentials were examined by Wound Healing assay and transwell assay. Results Human gastric carcinoma MGC803 cells exposed to DADS30 mg / L for 24h,the mRNA and protein expression level of Cath-D were significantly decreased( P < 0. 05),scar distance was widened( P < 0. 05),the number of cancer cells which passed through the Matrigel coated membrane was reduced( P < 0. 05); after Cath-D was interfered by siRNA,the mRNA and protein expression level of Cath-D were significantly decreased( P < 0. 05),scar distance was widened( P < 0. 05),the number of cancer cells which passed through the Matrigel coated membrane was reduced( P < 0. 05). Conclusions DADS can significantly inhibit the ability of migration and invasion ability of human gastric carcinoma MGC803 cells and its mechanism may be related to the expression of down-regulating Cath-D.更多还原