【摘要】 目的采用HPLC法同时测定防芷鼻咽颗粒中的丹皮酚、木兰脂素。方法采用Dikma Diamonsil C18色谱柱(250mm×4.6 mm,5μm),柱温30℃,以甲醇-0.5‰甲酸水溶液为流动相,梯度洗脱,流速1 mL·min-1,进样量10μL;丹皮酚、木兰脂素的检测波长分别为274、278 nm。结果丹皮酚8.203~265.5μg·mL-1、木兰脂素7.938~254.0μg·mL-1与峰面积的线性关系良好;平均加样回收率分别为98.59%、100.6%,RSD分别为1.43%、1.33%。结论所用方法简单、快速、可靠,可用于防芷鼻咽颗粒的质量控制。更多还原

【Abstract】 OBJECTIVE To develop a method for the simultaneous determination of paeonol and magnolin in Fangzhi nasopharyngeal granule by HPLC. METHODS The samples were separated on a Diamonsil C18column( 250 mm × 4. 6 mm,5 μm),by a gradient elution using methanol and 0. 5‰ aqueous formic acid as the mobile phase at a flow rate of 1 mL·min-1,the injection volume was10 μL. The column temperature was 30 ℃,and the detection wavelengths of paeonol was 274 nm,magnolin was 278 nm. RESULTS The calibration curves of paeonol and magnolin showed good linearity at the ranges of 8. 203-265. 5,7. 938-254. 0 μg·mL-1and the average recoveries were 98. 59% amd 100. 6% with the RSD of 1. 43% and 1. 33%,respectively. CONCLUSION The method is simple,rapid,reliable and can be used for the drug control.更多还原