【摘要】 通过对人工网箱养殖4年的斑鱾(Girella punctata Gray)亲鱼进行LHRH-A3激素催产,获得自然产卵的受精卵,于实验室中进行培育孵化,连续观察斑鱾胚胎发育的全过程。实验结果表明,斑鱾受精卵呈圆形,卵径0.882±0.041 mm、卵黄直径0.807±0.036 mm,具1个圆形油球,油球径为0.266±0.023 mm。在孵化水温23±0.5℃、盐度33.8条件下历时28 h 20 min完成整个胚胎发育过程,初孵仔鱼全长1.574±0.109mm。胚胎发育过程可划分为6个阶段,即受精卵、卵裂期、囊胚期、原肠胚期、神经胚期和器官形成期,包括27个发育时期。实验旨在了解斑鱾的胚胎发育规律,为开展斑鱾的人工繁殖提供基础资料。更多还原

【Abstract】 LRH-A 3 was injected into net-cage cultured Girella punctata Gray for spawning inducing. We conducted a consecutive survey on the embryonic development process of naturally fertilized zygotes,which were then cultivated and incubated in lab. The results showed that zygote of Girella punctata was round,the egg diameter was 0. 882 ± 0. 041 mm,yolk diameter was 0. 807 ± 0. 036 mm,and there was an oil ball( diameter 0.266 ±0.023 mm) in the yolk. The embryonic development process was finished under 23 ±0.5 ℃, at salinity 33. 8 for 28 h 20 min. The whole length of newly hatched larvae fish was 1. 574 ± 0. 109 mm. The embryonic development process of Girella punctata can be splited into 6 stages,including fertilized egg stage, cleavage stage,blastula stage,gastrula stage,neural stage and organogenesis stage,and it can also be divided into 27 periods. This study tried to understand the pattern of embryonic development process of Girella punctata,hoping to find some support for artificial reproduction.更多还原