【摘要】 目的:建立流苏石斛精制饮片中的石斛酚的含量测定方法。方法:采用Hypersil–C18(200mm×4.6mm,5μm)为色谱柱,流动相为乙腈-0.05%三氟乙酸(梯度洗脱),流速为1.0m L/min,检测波长为230nm。结果:石斛酚进样量在0.4~4.0μg范围内有良好的线性关系,r=0.9994,平均回收率99.65%,RSD为1.87%。结论:所建立方法操作简便、准确、可靠、重复性好,可用于流苏石斛精制饮片中石斛酚的含量测定。更多还原

【Abstract】 Objective: To establish a method for determining the content of dendrophenol in refined pieces of Dendrobium fimbriatum. Methods: High- performance liquid chromatography( HPLC) was performed on a Hypersil C18 chromatographic column( 200 mm × 4. 6mm,5μm) with a mobile phase of acetonitrile- 0. 05% trifluoroacetic acid( gradient elution) at a flow rate of 1. 0m L / min and a detection wavelength of 230 nm. Results: Dendrophenol showed a good linear relationship between sample size and peak area in the range of 0. 4- 4. 0μg( r = 0. 9994). The average recovery rate was 99. 65%,and the relative standard deviation was 1. 87%. Conclusion: The established HPLC method is simple,accurate,reliable,and repeatable and can be used for the determination of dendrophenol in refined pieces of Dendrobium fimbriatum.更多还原