【摘要】 目的:探讨葡萄籽原花青素对慢性间歇性低氧大鼠脑组织炎症反应及学习记忆能力的影响。方法:56只雄性SD大鼠随机分成对照组、模型组和葡萄籽原花青素高、低剂量干预组。对照组暴露于空气中,余3组制备慢性间歇性低氧模型(低氧处理6周)。葡萄籽原花青素高和低剂量干预组在低氧处理前2周开始每天1次灌胃葡萄籽原花青素200和100 mg·kg-1。低氧处理第2和6周,采用水迷宫实验测试大鼠学习记忆能力,电镜观察海马区神经细胞超微结构,免疫印迹法检测海马区脑组织磷酸化p38MAPK(p-p38MAPK)和IL-1β的表达。结果:与对照组比较,模型组神经细胞超微结构受损严重,脑组织p-p38MAPK和IL-1β表达升高,水迷宫实验逃避潜伏期延长、穿台次数减少(P<0.05)。与模型组比较,葡萄籽原花青素干预组神经细胞超微结构的损伤减轻,脑组织pp38MAPK和IL-1β表达水平降低,水迷宫实验逃避潜伏期缩短、穿台次数增多(P<0.05);高剂量干预组变化更为显著(P<0.05)。结论:葡萄籽原花青素可能通过减轻脑组织炎症反应,改善慢性间歇性低氧大鼠的学习记忆能力。更多还原

【Abstract】 Aim:To investigate the effect of grape seed proanthocyanidin on inflammation and learning memory capacity of rat with chronic intermittent hypoxia. Methods:A total of 56 male SD rats were randomly allocated into four groups:control group,model group,low and high dose of grape seed proanthocyanidin groups. Rats in control group were exposed in air,those in the other 3 groups suffered from intermittent hypoxia conditions for 6 weeks. 2 weeks before the hypoxia treatment,rats in low and high dose of grape seed proanthocyanidin groups were began to intragastricly administrate grape seed proanthocyanidin at dose of 100 and 200 mg·kg- 1,once per day,respectively. At the 2nd and 6th week of hypoxia treatment,neuron pathology in hippocampal region was observed using electron microscope,the expressions of phosphorylated p38MAPK and IL-1β proteins were detected by Western blot,and the learning memory capacity of rats were assessed by the Morris water maze. Results:Compared with those of control group,the microstructure of neuron was severely injured, the expressions of phosphorylated p38MAPK and IL-1β proteins significantly increased,and the escaping latency was prolonged,the frequency of crossing the platform decreased in the model group( P < 0. 05). Compared with those of the model group,neuron pathology changes significantly improved,phosphorylated p38MAPK and IL-1β expressions in brain tissue decreased,the escaping latency was shorter and the frequency of crossing the platform increased in the 2 grape seed proanthocyanidin groups( P < 0. 05),and the above mentioned changes in the high dose group were more significant( P < 0. 05). Conclusion:Grape seed proanthocyanidin could attenuate inflammation and improve learning memory capacity in rats with intermittent hypoxia.更多还原