【摘要】 目的:对高血压犬采用经导管射频消融去肾交感神经术验证其降压疗效,并检测术后神经损伤因子血浆S-100B及神经元特异性烯醇化酶(NSE)含量以探讨其与肾交感神经损伤的关系。方法:12条比格犬随机分为手术组(n=6)和假手术组(n=6)。采用高脂饮食建立高血压模型,手术组实施经导管射频消融去肾交感神经术,假手术组仅行肾动脉造影。观察血压变化,分别在术前和术后第3天、1周、2周、1个月、2个月、3个月监测血压并检测血浆S-100B和NSE的浓度。结果:建模后,手术组及假手术组收缩压(SBP)、舒张压(DBP)、平均动脉压(MBP)较基线均有显著上升(P<0.05)。经导管射频消融去肾交感神经术后,手术组术后1个月及3个月较假手术组SBP,DBP,MBP均有显著下降(P<0.05);术后3个月复查血肌酐正常,复查肾动脉造影未发现肾动脉狭窄。经导管射频消融去肾交感神经术后第3天、1周及2周手术组较假手术组血浆S-100B或NSE的含量升高(P<0.05)。结论:经导管射频消融去肾交感神经术可以显著和持久地降低高血压犬的SBP,DBP及MBP。经导管射频消融去肾交感神经术后S-100B及NSE血浆含量可能作为评估高血压犬肾交感神经损伤的指标。更多还原

【Abstract】 Objective: To observe the changes of blood pressure and S-100B, neuron specii c enolase(NSE) protein in hypertensive dogs with high fat diet at er catheter-based renal sympathetic denervation.Methods: Twelve Beagles were divided into an interventional group(n=6) and a sham-operation group(n=6). At er baseline measurements, the Beagles were fed with lard oil for 3 months. At er 3 months, the interventional group had renal sympathetic denervation by percutaneous catheterbased radiofrequency ablation and the control group had renal angiography. h e blood pressure, plasma S-100B, and NSE before the operation and 3 days, 1 week, 2 weeks, 1 month, 2 months and 3 months at er the operation were measured. Results: h e dogs had signii cantly higher levels of systolic blood pressure(SBP), diastolic blood pressure(DBP) and mean arterial pressure(MBP) compared to its baselines(P<0.05). h e SBP, DBP and MBP in the interventional group were signii cantly lower than those in the control group 1 month and 3 months after the operation(P<0.05). Three months after the operation, renal angiography in all dogs revealed no sign of renal artery stenosis. Plasma S-100B and NSE expression in the interventional group were higher than those in the control group 3 days, 1 week and 2 weeks at er the operation(P<0.05). Conclusion: Renal sympathetic denervation can signii cantly reduce the SBP, DBP and MBP in hypertensive dogs. h e plasma S-100B and NSE may be used as indicators for assessment of renal nerve injury at er renal sympathetic denervation.更多还原