【摘要】 目的探讨雷公藤红素对荷SHG44胶质瘤裸鼠移植瘤生长及血管生成的影响。方法 34只SHG-44胶质瘤裸鼠随机分为空白对照组、顺铂组、4mg/kg雷公藤红素组、2mg/kg雷公藤红素组和1mg/kg雷公藤红素组,每周两次,共给药4周。定期观察肿瘤生长情况测量肿瘤体积,计算抑瘤率。免疫组化染色检测移植瘤组织中的MVD、bFGF、VEGF、VEGFR1、VEGFR2的蛋白表达。结果 4mg/kg和2mg/kg的雷公藤红素能抑制肿瘤生长,下调移植瘤组织中VEGFR1、VEGFR2的蛋白表达和降低微血管计数(MVD),4mg/kg的雷公藤红素还能下调荷瘤裸鼠移植瘤组织中bFGF的蛋白表达。结论雷公藤红素能明显抑制荷SHG44胶质瘤裸鼠的移植瘤生长和血管生成,其机制可能与雷公藤红素下调肿瘤组织中bFGF、VEGFR1、VEGFR2的蛋白表达有关。更多还原

【Abstract】 Objective To investigate the effects of celastrol on the SHG44 glioma angiogenesis and growth of nude mouse bearing tumor.Methods 34SHG44 glioma of nude mouse were randomly divided into control group,cisplatin group,4mg/kg celastrol group,2mg/kg celastrol group and 1 mg/kg celastrol group.All mice were received ip five times weekly for four weeks.The xenografts dimensions were measured and the living states of mice observed.The expressions of microvessel density(MVD),basic fibroblast growth factor(bFGF)vascular endothelial growth factor(VEGF),vascular endothelial growth factor 1(VEGFR1),and vascular endothelial growth factor 2(VEGFR2)of transplantation tumor tissue were detected by immunohistochemistry staining.Results The dose of 4mg/kg and 2mg/kg celastrol could inhibit the xenografts growth,down-regulate the expression levels of VEGFR1 and VEGFR2and decrease MVD.The dose of 4mg/kg celastrol also could down-regulate the expression level of bFGF.Conclusion Celastrol can inhibit the SHG44 glioma angiogenesis and growth of nude mouse bearing tumor,which is correlated with down-regulating the expression levels of VEGFR1,VEGFR2 and bFGF.更多还原