【摘要】 从哥伦比亚生态型拟南芥(Arabidopsis thaliana Columbia 0)中克隆SnRK2.6[SNF1(sucrose non-fermenting-1)-related protein kinase 2.6]的完整编码序列(coding sequence,CDS),构建该基因的原核表达载体,将其转化BL21(DE3),经表达纯化得到SnRK2.6蛋白。激酶活性分析发现,原核表达纯化的SnRK2.6有自磷酸化和磷酸化MBP(myelin basin protein)的活性,为后续试验分析SnRK2.6的功能奠定基础。更多还原

【Abstract】 The whole coding sequence of SnRK2.6 [SNF1(sucrose non-fermenting-1)-related protein kinase 2.6]was cloned from Arabidopsis thaliana ecotype Columbia 0.The prokaryotic expression vector of the gene was constructed and transformed into Escherichia coli strain BL21(DE3)to obtain SnRK2.6protein by induction and purification.By activity analysis,the purified SnRK2.6had both autophosphorylation activity and phosphorylation activity of the myelin basic protein(MBP)substrate.This study lays the foundation for further study of the function of SnRK2.6.更多还原