【摘要】 以云南农业大学园林园艺学院选育的Ryau96172I和Ryau9327D两个番茄品系的花粉为试材,低温(4℃)及超低温(-196℃)条件下保存,接种于培养基上,置于28℃的培养箱进行恒温培养,镜检测定花粉活力。结果表明,离体花粉在4℃和-196℃条件下保存1~3 d,花粉活力与对照(25℃)相比没有显著差异;另外随着解冻次数的增多和储藏时间延长,花粉活力呈下降趋势,并于第7 d基本失去活力。更多还原

【Abstract】 The pollen of two tomato varieties, Ryau96172 I and Ryau9327 D, was adopted in our research. The two tomato varieties were bred by College of Landscape and Horticulture, Yunnan Agricultural University. The collected pollen was stored in low-temperature(4 ℃) and ultra-low-temperature(-196 ℃) circumstances.Then it was inoculated to the medium and cultured at 28 ℃ in thermostat incubator.The pollen viability was determined by electron microscope. The results showed that compared to that of pollen stored in control(25 ℃) circumstance, the viability of pollen stored in low-temperature(4 ℃) and ultra-low-temperature(-196 ℃) circumstances for 1-3 d did not change significantly. In addition, pollen viability trended to decrease with the increase of freeze-thaw cycle and storage time. The pollen lost basically the viability by the 7thd in the storage.更多还原