【摘要】 以9个菊花品种叶片、茎段为外植体,用6种分化培养基与3种生根培养基直接再生不定芽,研究激素组合及外植体对不同菊花品种直接再生的影响。结果表明,9个品种的茎段与6个品种的叶片都有较高的再生能力,但品种"28","5-17"的叶片未分化出不定芽,品种"11"的叶片再生率也不高;多数菊花品种茎段的再生能力高于叶片;再生率为84%~100%,繁殖系数为2.3~9.4,可应用于工厂化快繁与基因工程育种。更多还原

【Abstract】 Leaves and stems of 9 chrysanthemum species were taken as explants and inoculated to 6 differential media and root media to research effects of hormone combination and explants on chrysanthemum species. The results indicated that stem of 9 chrysanthemum species and leaf of 6 chrysanthemum species all showed higher regeneration capacity, with regeneration rate in 84%-100% and regeneration coefficient at 2.3-9.4. However, none adventitious buds were found differentiated from leaves of "28" and "5-17", and leaf regeneration capacity of "11" was lower, either. Most chrysanthemum species proved higher in stem regeneration capacity, instead of leaf. Therefore, chrysanthemum species can be applied for rapid reproduction and genetic engineering.更多还原