【摘要】 为了测定运输应激前后夏南牛血清中促肾上腺皮质激素、皮质醇、白介素-6、肿瘤坏死因子-α水平,观察其变化规律,试验选用8头体重相近的8月龄健康夏南牛,分别于运输前1,0小时,运输中2,4,7,10,20小时,运输后15,30小时及2,3,7,15,21天采集血液,分离血清,用放射免疫法测定各血清样品中血浆促肾上腺皮质激素、皮质醇、白细胞介素6、肿瘤坏死因子-α含量。结果表明:与运输前1小时相比,促肾上腺皮质激素在运输中2,4,10,20小时及运输后15小时极显著升高(P<0.01),在运输后30小时恢复到正常水平;皮质醇在运输过程中显著升高(P<0.05),在运输后2天恢复到正常水平;白介素-6在运输中4小时显著升高(P<0.05),在运输后15小时极显著升高(P<0.01),在运输后2天恢复到正常水平;肿瘤坏死因子-α在运输中6小时极显著升高(P<0.01),在运输后30小时恢复到正常水平。说明短时间运输应激会刺激免疫细胞因子促肾上腺皮质激素、皮质醇、白细胞介素6、肿瘤坏死因子-α水平增加,而过长时间的运输则会造成免疫抑制。更多还原

【Abstract】 To determine the levels of serum adrenocorticotropic hormone(ACTH),cortisol(COR),interleukin-6(IL-6) and tumor necrosis factor-alpha(TNF-α) in Xianan cattle before and after transportation stress. Eight healthy eight-month-old xianan cattle with similar weight were selected,and then their blood samples were collected to isolate serum at 0 h and 1 h before transportation; 2 h,4 h,7 h,10 h and20 h during transportation; 15 h,30 h,2 d,3 d,7 d,15 d and 21 d after transportation,respectively. The levels of plasma ACTH,COR,IL-6 and TNF-α from each serum sample were determined using radioimmunoassay. The results showed that,compared with 1 hour before transportation,the levels of ACTH were significantly increased(P < 0. 01) at 2 h,4 h,10 h and 20 h during transportation,and 15 h after transportation,and then returned to normal level at 30 h after transportation. The levels of COR during the whole transport process were significantly increased(P < 0. 05),then slowly returned to normal level at 2 d after transportation. The levels of IL-6 were significantly increased(P <0.05) at 4 h during transportation,and were extremely significantly increased(P <0.01) at 15 h after transportation,then returned to normal levels at 2 d after transportation. The levels of TNF-α were significantly increased(P < 0. 01) at 6 h during transportation,then returned to normal level at 30 h after transportation. The results indicate that short-term transportation stress can stimulate the levels of ACTH,COR,IL-6,and TNF-α as immune cytokines,but transportation over a long period of time will cause immunesuppression.更多还原