To establish an HPLC method for determination of paecniflorin of Bazhen decoction the assay was performed on a Symmetry C18column(250 mm × 4. 6 mm,5 μm). UV detector wavelength was at 230 nm. The mobile phase was acetonitrile-0. 05% phosphoric acid solution(15∶ 85). The flow rate was 1. 0 mL/min,and the column temperature was maintained at 30 ℃. The sampling volume was 20 μL. The calibration curve had good linear relationship in the range of 0. 014 ~ 0. 224 mg/mL,linear equation:Y = 40 798 281 X +2 855(r = ...