【摘要】 目的建立大鼠烟雾吸入性急性肺损伤动物模型。方法 24只雄性SD大鼠随机分成对照组及3h、6h、24h组,每组6只。对照组无烟雾吸入,3h、6h、24h组分别在烟雾吸入3、6、24 h后行血气分析及炎症因子、组织学检查。结果烟雾吸入组大鼠血气分析示氧合指数小于300,符合急性肺损伤诊断标准,炎症细胞因子较对照组明显升高(P<0.05);组织学检查示肺内出现肺水肿,炎症细胞浸润明显。结论通过烟雾发生装置给予大鼠吸入一定时间棉花制造的烟雾,可以成功制作烟雾吸入急性肺损伤动物模型。更多还原

【Abstract】 Objective To establish an acute lung injury model by cotton smoke inhalation in rats. Methods Twenty-four male SD rats were randomly allocated into control group,after smoke inhalation 3h,6h and 24 h group. The changes in blood gas analysis,inflammatory mediators such as interleukin 8( IL-8) were monitored in every group. Histopathology of lung in rats were observed.Results Compared with control group. PaO2 and PaCO2were significantly decreased in every smoke inhalation group( P < 0. 05). The increased of inflammatory mediators clew that lung injury were occurred after smoke inhalation in rats. Obvious edema,alveolar atrophy and inflammatory infiltration were found in smoke inhalation rats. Conclusion An acute lung injury model by cotton smoke inhalation in rats has been successfully established.更多还原