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高效土著花生根瘤菌筛选的初步研究

Preliminary study on screening of efficient indigenous peanut rhizobia

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【作者】 刘世旺刘建英徐艳霞徐君驰

【Author】 LIU Shi-wang;LIU Jian-ying;XU Yan-xia;XU Jun-chi;College of Life Science,Huanggang Normal University;Huanggang City Vegetable Administration Office;

【机构】 黄冈师范学院生命科学学院黄冈市蔬菜办公室

【摘要】 利用YMA培养基,从新鲜花生根瘤中分离得到黄冈土著花生根瘤菌6株,分别编号为HG-1、HG-2、HG-3、HG-4、HG-5、HG-6。选取黄冈地区广泛使用的3个花生品种(红花1号、红花2号、中花1号)对6个菌株进行盆栽回接实验,结果显示:接种根瘤菌的花生植株都有结瘤,所有菌株均为花生根瘤菌。通过比较根瘤数量和质量,初步筛选出与花生品种红花1号、红花2号、中花1号具有最佳共生固氮匹配关系的根瘤菌菌株分别是HG-5、HG-3、HG-2。

【Abstract】 6 strains of indigenous rhizobia in the area of Huanggang have been isolated from fresh peanut nodule extracts with YMA medium.They were separately named as HG-1,HG-2,HG-3,HG-4,HG-5 and HG-6.The isolated strains were inoculated back onto 3 varieties of peanut plants(Honghua-1,Honghua-2,Zhonghua-1)that are widely sowed in Huanggang.The results of the experiment in sand bottles show that all the peanut plants can nodulate after being inoculated with the strains,and all the isolated strains are peanut rhizobia.By comparing quantity and weight of nodule,it is known that the strain HG-5symbiotic nitrogen-fixing effect matches best with the peanut plant Honghua-1.So does HG-3with Honghua-2,and HG-2with Zhonghua-1.

【关键词】 根瘤菌花生共生固氮筛选
【Key words】 rhizobiumpeanutsymbiotic nitrogen-fixingscreening
【基金】 湖北省教育厅高校产学研合作资助项目(No.C2010061)资助
  • 【文献出处】 黄冈师范学院学报 ,Journal of Huanggang Normal University , 编辑部邮箱 ,2014年03期
  • 【分类号】S565.2;Q939.114
  • 【下载频次】123
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