【摘要】 植物次生细胞壁的发育受到一系列转录调控因子的调节.但关于这一过程如何与植物整体发育相整合,一直少有报道.拟南芥URO(UP-RIGHT ROSETTE)基因过量表达导致在相应突变体uro中自由态生长素浓度明显升高,并导致该突变体中次生细胞壁发育减弱.本研究利用突变体uro及其杂合子uro/+,通过定量PCR的方法,检测了一系列调控植物次生细胞壁分化相关基因的表达情况.结果表明,在uro/uro和uro/+中,植物次生细胞壁发育开关基因的表达受到明显抑制,而对于木质部导管细胞分化的开关基因没有明显影响.因此,URO基因是介导植物细胞壁次生加厚与植物生长发育状态关联的基因,URO基因通过抑制次生细胞壁发育达到维持细胞的生长状态的效果.更多还原

【Abstract】 There are several transcriptional factors that have been found taking part in secondary cell wall differentiation as master switches in Arabidopsis.However,how these master switches link to the major development event of plant is still unknown.Overexpression of URO(UPRIGHT ROSETTE) gene leads to much higher free IAA concentration in uro mutant than that in wild type.In this work,the expression levels of the master switch genes were checked in uro/uro and uro/ + mutants through quantity PCR reactions.The results show that expressions of the master switch genes were significantly repressed while the expression of xylem development master switcher genes were not effected when URO gene was over expressing in uro/uro and uro/ + mutants.It can be concluded that URO gene takes part in the integration of secondary cell wall differentiation with the major development process of plant and URO gene could keep plant cell in a growth manner through inhibition of the secondary cell wall development.更多还原