【摘要】 目的探讨改良愈疡方对右旋葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎(UC)模型小鼠肠道菌群的影响及治疗作用。方法 40只雄性C57BL/6小鼠按完全随机法分为正常组、模型组、治疗组、对照组各10只;正常组和模型组均用0.9%氯化钠注射液灌肠,治疗组给予改良愈疡方灌肠,对照组给予柳氮磺胺吡啶灌肠,共治疗14 d。观察结肠长度和质量变化,以及对UC小鼠细菌移位的影响。结果治疗组、对照组疾病活动指数(DAI)评分与模型组比较差异均有统计学意义(P<0.01);治疗组与对照组比较差异有统计学意义(P<0.05);治疗组与正常组比较差异无统计学意义(P>0.05)。饮用DSS后各组小鼠结肠长度均有不同程度缩短,治疗组与模型组比较差异有统计学意义(P<0.01),但与正常组相比较无统计学意义(P>0.05),对照组与模型组比较差异有统计学意义(P<0.01);各组小鼠结肠质量比较,治疗组与正常组、对照组、模型组比较差异均有统计学意义(P<0.01)。结肠长度与质量之比比较,模型组明显低于正常组(P<0.01),治疗组高于模型组和对照组(P<0.01,P<0.05),正常组与治疗组比较差异无统计学意义(P>0.05)。小鼠肠系膜淋巴结细菌移位率模型组明显高于正常组、对照组(P<0.01),治疗组未发现细菌移位,与正常组比较差异无统计学意义(P>0.05)。结论中药灌肠有助于维持UC模型小鼠的肠道正常菌群,对实验性模型小鼠结肠黏膜屏障具有保护作用。更多还原

【Abstract】 Objective To investigate the effect of modified Yuyang prescription on intestinal flora in mice model with ulcerative colitis induced by dextran sodium sulfate( DSS). Methods 40 male C57BL / 6 mice were randomly divided into normal group,model group,treatment group and control group,10 mice in each group; normal group and model group were given 0. 9% sodium chloride injection enema,enema of traditional Chinese medicine was used in treatment group,the control group was given sulfasalazine enema for 14d. The changes of colon length and quality and bacterial translocation in UC mice were observed. Results There was significant difference between treatment group,control group and model group on the score of disease activity index( DAI)( P < 0. 01). There was significant difference between treatment group and control group on the score of DAI( P < 0. 05). There was no difference between treatment group and normal group on the score of DAI( P > 0. 05). The colon length in each group was shorten in differ degrees after the treatment of DSS. There was significant difference between treatment group and model group on the colon length( P < 0. 01). But there was no difference between treatment group and normal group on the colon length( P > 0. 05). There was significant difference between control group and model group on the colon length( P < 0. 01). There was significant difference between treatment group,normal group,control group and model group on the colon quality( P < 0. 01). The ratio of colon length and quality in model group was lower than that in normal group( P < 0. 01). The ratio of colon length and quality in treatment group was higher than that in model and normal group( P < 0. 01,P < 0. 05). There was no difference between treatment group and normal group on the ratio of colon length and quality( P > 0. 05). Mesenteric lymph node bacterial translocation rate in model group was higher than in normal group and control group( P < 0. 01). There was no bacterial translocation in treatment group and there was no difference between treatment group and normal group( P > 0. 05). Conclusion Chinese medicine enema contributes to maintenance of the intestinal tract normal bacteria flora in mice model with ulcerative colitis,and has protection on colonic mucosal barrier of experimental model mice.更多还原