【摘要】 目的建立血浆中瑞芬太尼高效液相色谱测定方法 ,为临床合理用药提供依据。方法血浆样品用1-氯丁烷进行萃取,上清液提取至0.01 mol/L盐酸溶液中。分析柱为spherisorb-CN,流动相为0.02mol/L的NaH2PO4水溶液-乙腈(70:30),内含三乙胺0.01%,流速1.5mL/min,柱温为室温,紫外检测波长为210nm,进样量为200μL。选择择期行胸科手术患者8例,靶控输注瑞芬太尼浓度(Cp)6μg/L复合异丙酚2.5mg/kg,分别在瑞芬太尼靶控输注前及靶控输注后5、10、20、30、60、90min时抽取桡动脉血,用高效液相色谱法测定瑞芬太尼血药浓度(Cm)。结果人血浆中瑞芬太尼检测浓度在2.5~300μg/L范围内线性关系良好(r=0.999 1)。日内日间精密度均在10%以内,提取回收率大于78.57%,方法回收率在96.03%~104.67%。在靶控输注5~20min时,瑞芬太尼测定浓度低于靶控输注浓度(P<0.05),30~90min两者差异无统计学意义(P>0.05)。结论该方法操作简便,分离度好,其专属性、准确度、精密度、线性范围及稳定性均适合实际样品的检测。更多还原

【Abstract】 Objective High performance liquid chromarography（HPLC） method was established for determination of remifentanil in plasma.The study will provide academic foundation for the development and clinical use of remifentanil.Methods The samples extracted with 1-chlorobutane and supernatant was back extracted into 0.01 mol/L HC1 again.The separation was performed on a spherisorbCN column with a mobile phase of 0.02mol/L-l NaH₂PO₄-acetonitrile（70:30） including 0.01%triethylamine at a flow rate of 1.5mL/min.The column temperature was room temperature,the detectionwavelength was 210 nm and 200μL of solution was injected into HPLC.Eight patients undergoing thoracic surgery were assigned to receive remifentanil target controlled infusion（TCI）（6μg/L） and propofol intravenous injection（2.5mg/kg）.Arterial blood samples were taken before and after TCI remifentanil 5,10,20,30,60,90 min for measuring of blood concentration of remifentanil by HPLC.Results A linearity was obtained from 2.5 μg/L to 300μg/L of remifentanil in plasma with a good correlation（r = 0.999 1）.The between-day and within-day RSD%were less than 10%.The extraction recoveries were more than78.57%,and the method recoveries were between 96.03%and 104.67%.The determination of the concentration was lower than target plasma concentration at TCI 5 min to 20 min（P < 0.05） and there was no significant difference from 30 min to 90 min（P >0.05）.Conclusion This HPLC method is simple,sensitive and accurate,and it is suitable for routine determination of remifentanil levels in human plasma.更多还原