【摘要】 目的:探讨microRNA-429(miR-429)对人肺癌A549细胞增殖和凋亡的影响。方法:定量逆转录聚合酶链反应(qPCR)技术检测A549细胞瞬时转染miR-429mimic或inhibitor后miR-429的表达;通过MTT法和流式细胞术检测转染后细胞增殖能力和凋亡变化。结果:瞬时转染miR-429mimic 24h后,qPCR结果显示miR-429表达显著升高(9.3±0.3比1.0±0.1,P<0.05),而转染miR-429inhibitor可以显著降低miR-429表达(0.3±0.0比1.0±0.1,P<0.05);与对照组相比,过表达miR-429抑制肿瘤细胞增殖(2.8±0.2比5.0±0.3,P<0.05)并诱导凋亡(18.9±1.2比12.3±1.0,P<0.05),而下调miR-429表达促进肿瘤细胞增殖(6.3±0.4比5.0±0.3,P<0.05)并抑制凋亡(7.1±0.6比12.3±1.0,P<0.05)。结论:miR-429在肺癌中可能作为肿瘤抑制因子发挥功能。更多还原

【Abstract】 Objective:To investigate the effect of microRNA-429(miR-429)on the proliferation and apoptosis of lung cancer cells.Methods:We detected expression of miR-429after miR-429mimic and its inhibitor were transiently transfected into human lung cancer cell line A549.The cell proliferation and apoptosis after transfection were analyzed by MTT and flow cytometry.Results:24 h after miR-429 mimic transfection,the expression of miR-429 was significantly up-regulated(9.3±0.30 vs 1.0±0.08,P<0.05).However,miR-429inhibitor transfection led to downregulation of miR-429(0.3±0.05 vs 1.0±0.08,P<0.05).Compared with control transfection,miR-429overexpression inhibited tumor cell proliferation(2.8±0.21 vs 5.0±0.27,P<0.05) and induced cell apoptosis significantly(18.9±1.2 vs 12.3±1.0,P<0.05).On the contrary,downregulation of miR-429promoted tumor cell proliferation(6.3±0.35 vs 5.0±0.27,P< 0.05)and inhibited cell apoptosis significantly(7.1±0.6 vs 12.3±1.0,P<0.05).Conclusion:MiR-429may serve as a tumor suppressor and may be a potential therapeutic target in lung cancer.更多还原