【摘要】 为了了解鸭肝炎病毒的遗传变异情况,试验采用RT-PCR方法对采自广东省各地12个DHV临床分离株的VP1基因进行了扩增、克隆、测序和序列分析。结果表明,12个DHV分离株的VP1基因序列长度均为714 bp,编码238个氨基酸,12个分离株与Gen Bank公布的参考株核苷酸同源性为91.7%~95.8%,氨基酸同源性为94.5%~98.3%,而12个分离株之间的核苷酸序列同源性达到99.2%~100%,氨基酸同源性为97.9%~100%。系统分析证明,12个DHV分离株均为DHV-Ⅰ型,不同分离株VP1基因无碱基插入和缺失,但存在点突变,各DHV分离株间亲缘关系较近,但存在一定的遗传差异。更多还原

【Abstract】 In order to investigate the variation of Duck hepatitis virus(DHV), a total of 12 DHV VP1 genes from different regions of Guangdong province in China were amplified, cloned and sequenced. The results showed that the lengths of all VP1 sequences were 714 bp, encoding 238 amino acids. Homology analysis revealed that the VP1 genes of 12 isolates shared91.7%-95.8% nucleotide identity and 94.5%-98.3% amino acids identity with the reference strain of DHV from Gen Bank.Nucleotide sequence homology of VP1 gene among the 12 DHV isolates varied from 99.2% to 100%, while amino acid sequence homology varied from 97.9% to 100%. The phylogenetic analysis revealed that all the 12 isolates were DHV-Ⅰ,with no In Del, but having point mutations. All 12 isolates had a close genetic relationship with a few genetic differences.更多还原