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Ⅰ型鸭肝炎病毒VP1基因克隆与序列分析

Cloning and Sequence Analysis of VP1 Gene of Duck Hepatitis A

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【作者】 任邵娜袁生蒲文珺彭巧丽王辉陈志伟张浩吉李国清

【Author】 REN Shao-na;YUAN Sheng;PU Wen-jun;PENG Qiao-li;WANG Hui;CHEN Zhi-wei;ZHANG Hao-ji;LI Guo-qing;College of Veterinary Medicine,South China Agricultural University;Department of Veterinary Medicine,Foshan University;Shenzhen Laboratory,AIDS Institute,University of Hong Kong LKS Faculty of Medicine / Shenzhen Third People ’s Hospital;AIDS Institute,University of Hong Kong LKS Faculty of Medicine;

【机构】 华南农业大学兽医学院佛山科学技术学院动物医学系香港大学李嘉诚医学院艾滋病研究所深圳研究室深圳市第三人民医院香港大学李嘉诚医学院艾滋病研究所

【摘要】 为了了解鸭肝炎病毒的遗传变异情况,试验采用RT-PCR方法对采自广东省各地12个DHV临床分离株的VP1基因进行了扩增、克隆、测序和序列分析。结果表明,12个DHV分离株的VP1基因序列长度均为714 bp,编码238个氨基酸,12个分离株与Gen Bank公布的参考株核苷酸同源性为91.7%~95.8%,氨基酸同源性为94.5%~98.3%,而12个分离株之间的核苷酸序列同源性达到99.2%~100%,氨基酸同源性为97.9%~100%。系统分析证明,12个DHV分离株均为DHV-Ⅰ型,不同分离株VP1基因无碱基插入和缺失,但存在点突变,各DHV分离株间亲缘关系较近,但存在一定的遗传差异。

【Abstract】 In order to investigate the variation of Duck hepatitis virus(DHV), a total of 12 DHV VP1 genes from different regions of Guangdong province in China were amplified, cloned and sequenced. The results showed that the lengths of all VP1 sequences were 714 bp, encoding 238 amino acids. Homology analysis revealed that the VP1 genes of 12 isolates shared91.7%-95.8% nucleotide identity and 94.5%-98.3% amino acids identity with the reference strain of DHV from Gen Bank.Nucleotide sequence homology of VP1 gene among the 12 DHV isolates varied from 99.2% to 100%, while amino acid sequence homology varied from 97.9% to 100%. The phylogenetic analysis revealed that all the 12 isolates were DHV-Ⅰ,with no In Del, but having point mutations. All 12 isolates had a close genetic relationship with a few genetic differences.

【关键词】 Ⅰ型鸭肝炎病毒VP1基因克隆序列分析
【Key words】 DHV-ⅠVP1 genecloningsequence analysis
  • 【文献出处】 湖北农业科学 ,Hubei Agricultural Sciences , 编辑部邮箱 ,2014年21期
  • 【分类号】S852.65
  • 【被引频次】3
  • 【下载频次】114
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