【摘要】 在盆栽条件下,以狭叶浙贝母(Fritillaria thunbergii Miq.)为材料,研究了浙贝母花期高温胁迫条件下表油菜素内酯(EBR)对浙贝母耐热性和光合特性的影响。结果表明,0.10、0.50 mg/L EBR能明显提高叶片中可溶性蛋白含量、SOD、POD和CAT活性,降低MDA含量,增强其抗氧化能力,缓解高温胁迫导致的膜质过氧化伤害;同时,EBR提高了叶片叶绿素含量、Pn、Gs和Tr,从而提高了高温胁迫下浙贝母植株的光合作用;然而EBR并不能提高其Pro及可溶性糖含量,对叶片中类胡萝卜素及胞间CO2浓度(Ci)影响差异不大。结果表明,EBR通过提高高温胁迫下浙贝母叶片可溶性蛋白及叶绿素含量、抗氧化酶活性,来降低体内活性氧水平,维持较高的光合速率,以0.50 mg/L EBR效果最好。更多还原

【Abstract】 The effects of epibrassionlide on thermotolerance and photosynthesis in leaves of F. thunbergii(xiaye) grown in pots were investigated under high temperature. The results showed that the treatment of 0.10,0.50 mg / L-12.4-epibrassionlide could effectively enhance antioxidation ability and alleviate injury of membrane lipid peroxidation caused by high temperature through increasing content of soluble protein, activities of superoxide dismutase(SOD),peroxidase(POD) and catalase(CAT),and decreasing malondialdehyde(MDA) content. EBR pretreatment improved the photosynthesis in F. thunbergii(xiaye)leaves by increasing the chlorophyll content, net photosynthetic rate(Pn),stomatal conductance(Gs),ranspiration rate(Tr). EBR pretreatment couldn’t increase contents of proline and soluble sugar, and had little effect on carotenoid content and intercellular CO2concentration(Ci) in leaves. It is indicated that EBR pretreatment could suppress reactive oxygen levels,keep higher photosynthetic rate by increasing contents of soluble protein and chlorophyll, activities of antioxidant enzymes.The better alleviating effect on high temperature damage was 0.50 mg / L EBR.更多还原