【摘要】 采用单因素试验与正交试验相结合优化小叶黑柴胡(Bupleurum smithii Wolff var.parvifolium Shan et Y.Li)总皂苷的提取工艺,考察了提取温度、提取溶剂pH、乙醇浓度、料液比(g/mL,下同)、提取次数和提取时间对小叶黑柴胡总皂苷得率的影响。结果表明,小叶黑柴胡总皂苷的较优提取工艺条件为料液比1∶10,乙醇浓度80%,提取次数3次,提取时间1.0 h,提取温度80℃,提取溶剂pH为9。该工艺条件下,小叶黑柴胡总皂苷的得率为2.78%。验证试验表明该工艺稳定可行,可为小叶黑柴胡的深入研究提供参考。更多还原

【Abstract】 The technology of extracting total saponins from Bupleurum smithii Wolff var. parvifolium Shan et Y. Li were optimized by single-factor test and orthogonal design factors such as extraction temperature, pH of solvent, extraction times, ethanol concentration and liquid ratio were investigated. The results showed that the yield of total saponins from Bupleurum smithii Wolff var. parvifolium Shan et Y. Li. was 2.78% when it was extracted three times with each time 1 hour under 80 ℃, ethanol concentration of 80%, solid to liquid ratio of 1∶10(g / mL) and pH of 9. The technology was proved to be reliable and could be used as a reference for utilizing of Bupleurum smithii Wolff var. parvifolium Shan et Y. Li.更多还原