【摘要】 以地衣芽孢杆菌(Bacillus licheniformis)WX-02为研究对象,以提高其发酵产生的聚γ-谷氨酸的产量为研究目的,经摇瓶发酵得到优化的硝酸钠补料方案,即初始硝酸钠浓度为10 g/L,发酵至12 h时补加5 g/L硝酸钠时聚γ-谷氨酸产量最大。将此补料方案在3 L发酵罐上进行验证。结果表明,发酵得到的菌体生物量和聚γ-谷氨酸产量最大值分别为4.5 g/L和38.7 g/L,与对照相比分别提高55.2%和14.8%。通过补硝酸钠可以提高菌体生物量和对谷氨酸的利用率,从而提高聚γ-谷氨酸的发酵水平。更多还原

【Abstract】 Bacillus licheniformis WX-02 was used to study the ways of improving poly(γ-glutamic acid)(γ-PGA) yield. The optimal conditions of sodium nitrate feeding for γ-PGA fermentation by Bacillus licheniformis WX-02 by flask-shaking fedbatch fermentation were the initial sodium nitrate concentration 10 g / L, and 5 g / L of sodium nitrate added when fermentation to 12 h. The fermentation was carried out in a 3 L fermentor, showing that the maximum of biomass and γ-PGA yield was 4.5 g / L and 38.7 g / L with increase of 55.2% and 14.8%, compared with the control. It is indicated that adding sodium nitrate can increase biomass, enhance the utilization of glutamate, and improve the γ-PGA fermentation.更多还原