【摘要】 目的从茅苍术分离纯化出多糖APW1a,研究其组成及初步结构。方法茅苍术根茎经水提醇沉、脱蛋白、透析,DEAE纤维素与Sephadex G-100凝胶柱洗脱得APW1a;采用紫外、高效凝胶液相、气相、红外、核磁共振等色谱分析其组成及初步结构。结果 APW1a由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖组成,物质的量比为0.16∶0.17∶0.24∶0.55∶0.89∶1分子量约为5.3×103D,糖环以β型吡喃糖为主,并有甲基、酯酰等基团。结论分析了茅苍术多糖APW1a的组成及初步结构,为茅苍术多糖的进一步的研究提供了基础。更多还原

【Abstract】 OBJECTIVE To study the isolation,the composition and preliminary characterization of polysaccharide APW1 a from Atractylodes lancea( Thunb.) DC. METHODS The crude polysaccharides were obtained by extracting with boiling-water and precipitating with alcohol,the protein was removed by TCA,The cleared polysaccharide were purified by DEAE-cellulose,Sephadex G-100 column,received APW1 a. The composition and characterization of APW1 a was analyzed by UV,HPLC,GC,IR,1H-NMR. RESULTS APW1 a consistsed of Rha、Ara、Xyl、Man、Glu、Gal in a molar ratio of 0. 16∶0. 17∶0. 24∶0. 55∶0. 89∶1. The molecular mass was 5. 3 × 103 D. The structure was analyzed by chromatography suggesting APW1 a was in the form of pyranose β-glycosidic bond mainly,and it contained methyl group,ester acyl group and so on. CONCLUSION The structure of APW1 a is first discussed,these work cuold establish foundation for the further study of Atractylodes Polysaccharides.更多还原